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MB Sample ID: SA010803

Local Sample ID:Treated Cecal Content
Subject ID:SU000244
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J; Wild Type and Ahr-/-
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

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Subject:

Subject ID:SU000244
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J; Wild Type and Ahr-/-
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
Treated Cecal ContentSA010803FL002574Malegender
Treated Cecal ContentSA010803FL002574Tcdf Treatedgenotype

Collection:

Collection ID:CO000232
Collection Summary:-
Sample Type:Feces, liver, cecal contents, and intestine

Treatment:

Treatment ID:TR000252
Treatment Summary:Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days
Treatment:Dietary
Treatment Compound:TCDF
Treatment Route:Oral
Treatment Dose:24 µg/kg body weight

Sample Preparation:

Sampleprep ID:SP000246
Sampleprep Summary:-
Sampleprep Protocol Filename:NMR_metabolomics_protocol.docx
Processing Method:Homogenization
Processing Storage Conditions:-20
Extraction Method:Methanol/Water
Extract Concentration Dilution:2 Methanol : 1 Water
Sample Resuspension:D2O
Sample Spiking:TSP-d4
Organ:Liver, small and large intestine

Analysis:

MB Sample ID:SA010803
Analysis ID:AN000335
Analysis Type:NMR
Chromatography ID:CH000251
Num Factors:2

NMR:

NMR ID:NM000054
Analysis ID:AN000335
Instrument Name:Bruker Avance III
Instrument Type:FT-NMR
NMR Experiment Type:NOESY PR1D
Spectrometer Frequency:600 MHz
NMR Solvent:D2O
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