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MB Sample ID: SA010889
Local Sample ID: | 103 |
Subject ID: | SU000252 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000252 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
103 | SA010889 | FL002646 | AHR -/- | Strain |
103 | SA010889 | FL002646 | Control | Treatment |
Collection:
Collection ID: | CO000242 |
Collection Summary: | Feces and urine were collected every other day. Then Liver, blood and intestinal tissue were collected after death |
Sample Type: | Feces, Urine, Liver, Blood, Intestinal tissue |
Treatment:
Treatment ID: | TR000262 |
Sample Preparation:
Sampleprep ID: | SP000256 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | GC_Sample_Prep.docx |
Processing Method: | Homogenization |
Extraction Method: | Methanol-Chloroform |
Extract Enrichment: | centerfugation |
Extract Cleanup: | bottom layer was dried with N2 gas, resuspended in MEOH/HCL and then hexane and saline, top layer was collected |
Sample Resuspension: | resuspended in hexane |
Sample Spiking: | C15:0 free acid and methy ester of C17:0 at 5 ?L |
Organ: | Liver |
Combined analysis:
Analysis ID | AN000349 | AN000350 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | GC | GC |
Chromatography system | ||
Column | ||
MS Type | EI | EI |
MS instrument type | Single quadrupole | Single quadrupole |
MS instrument name | Agilent 5975C | Agilent 5975C |
Ion Mode | POSITIVE | POSITIVE |
Units | Peak area | Peak area |
Chromatography:
Chromatography ID: | CH000260 |
Chromatography Type: | GC |
MS:
MS ID: | MS000295 |
Analysis ID: | AN000349 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |
MS ID: | MS000296 |
Analysis ID: | AN000350 |
Instrument Name: | Agilent 5975C |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |