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MB Sample ID: SA011496
Local Sample ID: | CC267_EBSS2 |
Subject ID: | SU000271 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Animal Animal Supplier: | inhouse breeding |
Cell Biosource Or Supplier: | BM derived B cells |
Cell Strain Details: | wt and EuMyc |
Cell Primary Immortalized: | primary |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000271 |
Subject Type: | Animal cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Animal Animal Supplier: | inhouse breeding |
Cell Biosource Or Supplier: | BM derived B cells |
Cell Strain Details: | wt and EuMyc |
Cell Primary Immortalized: | primary |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
CC267_EBSS2 | SA011496 | FL002868 | wt | Treatment |
CC267_EBSS2 | SA011496 | FL002868 | EBSS | media |
Collection:
Collection ID: | CO000262 |
Collection Summary: | Cells were counted, washed with cold PBS and then flash-frozen in liquid N2 |
Sample Type: | cells |
Volumeoramount Collected: | 0.5.00E+07 cells |
Storage Vials: | screw caps |
Treatment:
Treatment ID: | TR000282 |
Treatment Summary: | Cells were incubated for 2hrs in EBSS at 37C. |
Cell Media: | 45%DMEM, 45%IMDM, 10%FCS, Q, PS, Sodium Pyruvate, beta mercaptoethanol and EBSS with 10% dialized FBS |
Cell Harvesting: | washed, and pellet flashfroozen in liquid N2 |
Cell Pct Confluence: | 2xE06 per ml |
Sample Preparation:
Sampleprep ID: | SP000276 |
Sampleprep Summary: | Amino acid |
Combined analysis:
Analysis ID | AN000397 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters AccQ Tag (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | pmol/million cells |
Chromatography:
Chromatography ID: | CH000281 |
Chromatography Summary: | Amino Acid |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000341 |
Analysis ID: | AN000397 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |