Return to study ST000251 main page
MB Sample ID: SA011498
| Local Sample ID: | CC267_EBSS1 |
| Subject ID: | SU000271 |
| Subject Type: | Animal cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Animal Animal Supplier: | inhouse breeding |
| Cell Biosource Or Supplier: | BM derived B cells |
| Cell Strain Details: | wt and EuMyc |
| Cell Primary Immortalized: | primary |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000271 |
| Subject Type: | Animal cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Animal Animal Supplier: | inhouse breeding |
| Cell Biosource Or Supplier: | BM derived B cells |
| Cell Strain Details: | wt and EuMyc |
| Cell Primary Immortalized: | primary |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| CC267_EBSS1 | SA011498 | FL002868 | wt | Treatment |
| CC267_EBSS1 | SA011498 | FL002868 | EBSS | media |
Collection:
| Collection ID: | CO000262 |
| Collection Summary: | Cells were counted, washed with cold PBS and then flash-frozen in liquid N2 |
| Sample Type: | cells |
| Volumeoramount Collected: | 0.5.00E+07 cells |
| Storage Vials: | screw caps |
Treatment:
| Treatment ID: | TR000282 |
| Treatment Summary: | Cells were incubated for 2hrs in EBSS at 37C. |
| Cell Media: | 45%DMEM, 45%IMDM, 10%FCS, Q, PS, Sodium Pyruvate, beta mercaptoethanol and EBSS with 10% dialized FBS |
| Cell Harvesting: | washed, and pellet flashfroozen in liquid N2 |
| Cell Pct Confluence: | 2xE06 per ml |
Sample Preparation:
| Sampleprep ID: | SP000276 |
| Sampleprep Summary: | Amino acid |
Combined analysis:
| Analysis ID | AN000397 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 Infinity |
| Column | Waters AccQ Tag (100 x 2mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ |
| Ion Mode | POSITIVE |
| Units | pmol/million cells |
Chromatography:
| Chromatography ID: | CH000281 |
| Chromatography Summary: | Amino Acid |
| Methods Filename: | SB_AA_Assay.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.7 mL/min |
| Solvent A: | AccQ·Tag Eluent A |
| Solvent B: | accQ·Tag Eluent B |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000341 |
| Analysis ID: | AN000397 |
| Instrument Name: | Agilent 6490 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
