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MB Sample ID: SA011674
Local Sample ID: | Urine19 |
Subject ID: | SU000276 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Mean (+SD): 54+8 years |
Weight Or Weight Range: | Mean (+SD): 76+11 kg; BMI < 30 |
Gender: | males and females (see study design) |
Human Race: | Caucasian (C) and African American (AA) |
Human Medications: | None |
Human Prescription Otc: | None |
Human Smoking Status: | Non-smokers |
Human Alcohol Drug Use: | None |
Human Inclusion Criteria: | Healthy, non-smoking, medication free adults |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000276 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Mean (+SD): 54+8 years |
Weight Or Weight Range: | Mean (+SD): 76+11 kg; BMI < 30 |
Gender: | males and females (see study design) |
Human Race: | Caucasian (C) and African American (AA) |
Human Medications: | None |
Human Prescription Otc: | None |
Human Smoking Status: | Non-smokers |
Human Alcohol Drug Use: | None |
Human Inclusion Criteria: | Healthy, non-smoking, medication free adults |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Urine19 | SA011674 | FL002912 | M | Gender |
Urine19 | SA011674 | FL002912 | C | Race |
Collection:
Collection ID: | CO000268 |
Collection Summary: | - |
Collection Protocol ID: | Clean catch urine collection |
Collection Protocol Filename: | SOP_for_urine_sample_collection |
Sample Type: | Urine |
Collection Method: | clean catch into NaN3 coated collection cups |
Collection Location: | Michigan Clinical Research Unit |
Collection Frequency: | 1 |
Collection Time: | Fasting, between 0830-0930 |
Volumeoramount Collected: | 30-50ml |
Storage Conditions: | Initial 4C then -80C following processing |
Collection Vials: | Urine collection cup |
Collection Tube Temp: | RT |
Additives: | NaN3 |
Treatment:
Treatment ID: | TR000288 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000282 |
Sampleprep Summary: | - |
Sampleprep Protocol Filename: | SOP_for_urine_sample_processing |
Processing Method: | Centrifugation |
Processing Storage Conditions: | 4C |
Extraction Method: | N/A |
Extract Concentration Dilution: | N/A |
Extract Enrichment: | N/A |
Extract Cleanup: | N/A |
Extract Storage: | N/A |
Sample Resuspension: | N/A |
Analysis:
MB Sample ID: | SA011674 |
Analysis ID: | AN000405 |
Laboratory Name: | University of Alberta National High Field Nuclear Magnetic Resonance Centre |
Analysis Type: | NMR |
Software Version: | VNMRJ 2.2c |
Operator Name: | Ryan McKay |
Chromatography ID: | CH000286 |
Num Factors: | 3 |
Num Metabolites: | 59 |
MB Sample ID: | SA011674 |
Analysis ID: | AN000404 |
Laboratory Name: | University of Michigan Biochemical NMR Core Laboratory |
Analysis Type: | NMR |
Software Version: | VNMRJ 3.2 |
Operator Name: | Michael Finkel |
Chromatography ID: | CH000286 |
Num Factors: | 3 |
Num Metabolites: | 59 |
NMR:
NMR ID: | NM000056 |
Analysis ID: | AN000404 |
Instrument Name: | Agilent 500/54 VNMRS |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Shimming Method: | Healthy urine quantified metabolite data |
Receiver Gain: | 10% (~0.5mM) |
Offset Frequency: | 500 MHz |
Presaturation Power Level: | ONE-Probe |
Chemical Shift Ref Cpd: | D2O |
Temperature: | 5mm |
Number Of Scans: | Auto shim (gradient shimming), and manual when necessary. |
Dummy Scans: | 1D-NOESY |
Acquisition Time: | saturation at 80 Hz induced field strength |
Relaxation Delay: | 5.5ms |
Real Data Points: | around -178Hz |
Zero Filling: | DSS-d6 |
Apodization: | 25C |
NMR ID: | NM000057 |
Analysis ID: | AN000405 |
Instrument Name: | Oxford 600 |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D 1H |
Shimming Method: | Healthy urine quantified metabolite data |
Receiver Gain: | 10% (~0.5mM) |
Offset Frequency: | 600 MHz |
Presaturation Power Level: | HX probe with Z-axis gradient coils |
Chemical Shift Ref Cpd: | D2O |
Temperature: | 5mm |
Number Of Scans: | Auto shim (gradient shimming), and manual when necessary. |
Dummy Scans: | 1D-NOESY |
Acquisition Time: | saturation at 80 Hz induced field strength |
Relaxation Delay: | 5.5ms |
Real Data Points: | around -178Hz |
Zero Filling: | DSS-d6 |
Apodization: | 25C |