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MB Sample ID: SA014739
Local Sample ID: | F_colon |
Subject ID: | SU000347 |
Subject Type: | Animal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000347 |
Subject Type: | Animal |
Subject Species: | Canis lupus familiaris |
Taxonomy ID: | 9615 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
F_colon | SA014739 | FL003325 | Colon | Organ |
Collection:
Collection ID: | CO000341 |
Collection Summary: | Samples were collected from dogs euthanized for an unrelated reason. From 6 different dogs, samples were taken from 4 different sites along the gastrointestinal tract (callingthese treatments). Samples were frozen at 80, then thawed to aliquot into 100mg portions, and refrozen at 80 and shipped on dry ice. No other preparation/additives. |
Collection Protocol Comments: | StudyDesign-JuliaHonneffer-101614.pdf |
Sample Type: | Duodenum |
Treatment:
Treatment ID: | TR000361 |
Treatment Summary: | Different sites along the gastrointestinal tract (duodenum, ileum, colon, rectum). |
Treatment Protocol Filename: | StudyDesign-JuliaHonneffer-101614.pdf |
Sample Preparation:
Sampleprep ID: | SP000355 |
Sampleprep Summary: | - |
Combined analysis:
Analysis ID | AN000521 | AN000522 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Unspecified | Unspecified |
Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Agilent 6530 QTOF | Agilent 6550 QTOF |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak height | Peak height |
Chromatography:
Chromatography ID: | CH000365 |
Chromatography Summary: | UPLC |
Methods Filename: | Data_Dictionary_Fiehn_laboratory_HILIC_QTOF_MS.pdf |
Instrument Name: | Unspecified |
Column Name: | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
Column Pressure: | 200-700 bar |
Column Temperature: | 45 C |
Flow Gradient: | 100% B to 30% B |
Flow Rate: | 0.4 mL/min |
Internal Standard: | See data dictionary |
Retention Time: | See data dictionary |
Sample Injection: | 3 uL |
Analytical Time: | 14 min |
Capillary Voltage: | 4500 |
Time Program: | 16.75 min |
Weak Wash Solvent Name: | 1:1 ACN:H2O |
Weak Wash Volume: | 5 seconds |
Strong Wash Solvent Name: | 1:1 ACN:H2O |
Target Sample Temperature: | Autosampler temp 4 C |
Randomization Order: | Excel |
Chromatography Type: | HILIC |
MS:
MS ID: | MS000457 |
Analysis ID: | AN000521 |
Instrument Name: | Agilent 6530 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
MS Comments: | Lipidomics runs are MS1 data; Identifications made from pooled MS/MS runs |
Ion Mode: | POSITIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 |
Ion Spray Voltage: | 1000 |
Ionization: | Pos |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | Extended Dynamic Range |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |
MS ID: | MS000458 |
Analysis ID: | AN000522 |
Instrument Name: | Agilent 6550 QTOF |
Instrument Type: | QTOF |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Capillary Voltage: | 3500 |
Collision Gas: | Nitrogen |
Dry Gas Flow: | 8 l/min |
Dry Gas Temp: | 325 |
Fragment Voltage: | 120 |
Fragmentation Method: | Auto MS/MS |
Ion Source Temperature: | 325 |
Ion Spray Voltage: | 1000 |
Ionization: | Pos |
Precursor Type: | Intact Molecule |
Reagent Gas: | Nitrogen |
Source Temperature: | 325 C |
Desolvation Gas Flow: | 11 l/min |
Desolvation Temperature: | 350 C |
Nebulizer: | 35 psig |
Octpole Voltage: | 750 |
Resolution Setting: | Extended Dynamic Range |
Scan Range Moverz: | 60-1700 Da |
Scanning Cycle: | 2 Hz |
Scanning Range: | 60-1700 Da |
Skimmer Voltage: | 65 |