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MB Sample ID: SA015122
| Local Sample ID: | Allogeneic 4Spleen |
| Subject ID: | SU000358 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000358 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammal |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Allogeneic 4Spleen | SA015122 | FL003367 | Spleen | Site |
Collection:
| Collection ID: | CO000352 |
| Collection Summary: | Female C57BL/6 (I-Ab; CD45.2+), BALB/c (H2d), and C3H.sw (H2b) mice were purchased from National Cancer Institute. The age of mice used for experiments ranged between 7 and 12 weeks. |
| Sample Type: | Blood |
Treatment:
| Treatment ID: | TR000372 |
| Treatment Summary: | BMTs were performed as previously described {Reddy:2005dc}{Reddy:2008kl}. Briefly, syngeneic (BALB/c ? BALC/b or C57BL/6 ? C57BL/6) and allogeneic (C57BL/6 ? BALB/c or C3H.sw ? C57BL/6) recipients received lethal irradiation. On day -1, BALB/c recipients received a total of 800 cGy of irradiation (split dose separated by 3 hours) and B6 animals received a single dose of 1000 cGy. Donor splenic CD90.2+ T cells were magnetically separated using an autoMACs (Miltenyi Biotec; Bergisch Gladbach, Germany) and 0.5 x 106 – 1 x 106 T cells were transferred to BALB/c recipients and 2 x 106 T cells were transferred to C57BL/6 recipients. 5 x 106 donor whole bone marrow was transferred to all recipients. |
Sample Preparation:
| Sampleprep ID: | SP000365 |
| Sampleprep Summary: | To determine targeted fatty acid quantitation, samples (plasma, spleen, liver, intestine, and intestinal fecal content) from mice 7d and 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Homogenized tissues were normalized to their protein content, while 50µL of plasma was used per sample and Equal volumes of plasma and homogenized tissue were utilized, while50 mg fecal content was weighed at necropsy before extraction. Samples were dispersed in acidified water spiked with stable isotope-labeled SCFA standards and extracted with diethyl ether. |
Combined analysis:
| Analysis ID | AN000549 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 6890N |
| Column | Phenomenex ZB-WAX |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5973 |
| Ion Mode | POSITIVE |
| Units | mmol/mg |
Chromatography:
| Chromatography ID: | CH000386 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Phenomenex ZB-WAX |
| Chromatography Type: | GC |
MS:
| MS ID: | MS000485 |
| Analysis ID: | AN000549 |
| Instrument Name: | Agilent 5973 |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
