Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA020082

Local Sample ID:	B_75
Subject ID:	SU000431
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Subject:

Subject ID:	SU000431
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
B_75	SA020082	FL006519	Oxytocin	Treatment
B_75	SA020082	FL006519	Cerb	Brain region

Collection:

Collection ID:	CO000425
Collection Summary:	24 hr after the social test, mice were deeply anesthetized with 5% isoflurane, followed by rapid decapitation. Brains were removed, rinsed with ice-cold water, and then rapidly dissected into the following 5 regions: forebrain, midbrain, cerebellum, hindbrain, and olfactory bulbs. The dissected parts were flash-frozen on dry ice and stored at -80o C. Samples were coded by treatment: vehicle-treated mice (n=8) and OT-treated mice (n=12).
Sample Type:	Brain

Treatment:

Treatment ID:	TR000445
Treatment Summary:	BALB/cByJ male mice (4-5 weeks in age; Jackson Laboratory, Bar Harbor, ME) were given four treatments of either vehicle or OT (1.0 mg/kg; IP) across 8 days, with at least 48 hours between each injection. Mice were evaluated in a 3-chamber choice test for social preference 24 hr after the final injection.

Sample Preparation:

Sampleprep ID:	SP000438
Sampleprep Summary:	The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.

Sampleprep ID:

SP000438

Sampleprep Summary:

The NMR samples were used for the neurotransmitter analysis after NMR data acquisition. These samples were lyophilized overnight followed by reconstitution in 50 µL 95:5 methanol:water. Following a final centrifugation, extracts were transferred to vials for analysis by injection (20 µL) onto the Thermo Scientific (TS) 3000 series U-HPLC system. Compound separation was achieved on a Thermo Scientific Hypersil Gold, 200 x 2.1 mm column, and 1.9 µm particle size with an isocratic flow rate of 0.400 mL/min with a column temperature of 30°C. Simultaneous detection of neurotransmitters was achieved by the TS 5600A 16 channel coulometric array ECD with coulometric cells set at incremental potentials ranging from -150 mV to 900 mV. A Standard Mix containing 14 target compounds at known concentrations was analyzed just prior to each batch to confirm the retention time and elution channel of each compound. Additionally, the mix was used as an external standard to semi-quantitate any targets found in the extracts.

Analysis:

MB Sample ID:	SA020082
Analysis ID:	AN000650
Analysis Type:	NMR
Num Factors:	11
Num Metabolites:	14
Units:	ng

NMR:

NMR ID:	NM000072
Analysis ID:	AN000650
Instrument Name:	Thermo Scientific 5600A 16 channel coulometric array ECD
Instrument Type:	Other
NMR Experiment Type:	Other