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MB Sample ID: SA021031
Local Sample ID: | S_7 |
Subject ID: | SU000439 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male/Female |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000439 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male/Female |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
S_7 | SA021031 | FL005275 | NCF | Group |
Collection:
Collection ID: | CO000433 |
Collection Summary: | Human saliva was collected from patients with Cystic Fibrosis along with their heterozygous non-CF mothers,and non-CF control subjects. |
Sample Type: | Saliva |
Treatment:
Treatment ID: | TR000453 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP000446 |
Sampleprep Summary: | Aliquots of 150 µL human saliva were mixed with 0.600 mL of degassed 3:3:2 Acetonitrile:Isopropanol:Water solution by vortex mixer then centrifuged. Supernatant recovered and evaporated to dryness on speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
Analysis ID | AN000660 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus 4D GC |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | Intensity |
Chromatography:
Chromatography ID: | CH000477 |
Instrument Name: | Leco Pegasus 4D GC |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Column Pressure: | 12.8psi |
Flow Gradient: | constant flow |
Flow Rate: | 1ml/min |
Injection Temperature: | 250C |
Retention Index: | FAME markers C8-C30 |
Chromatography Type: | GC |
MS:
MS ID: | MS000586 |
Analysis ID: | AN000660 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |