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MB Sample ID: SA022819
Local Sample ID: | 203 |
Subject ID: | SU000471 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Males with CFS were 53 (±2.8) y old (mean±SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y). |
Gender: | 23 females and 22 males with CFS and 18 male and 21 female controls. |
Human Ethnicity: | White/non-Hispanic |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000471 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Males with CFS were 53 (±2.8) y old (mean±SEM; range, 21–67 y). Females were 52 (±2.5) y old (range, 20–67 y). |
Gender: | 23 females and 22 males with CFS and 18 male and 21 female controls. |
Human Ethnicity: | White/non-Hispanic |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
203 | SA022819 | FL005563 | CFS | Disease |
203 | SA022819 | FL005563 | Male | gender |
Collection:
Collection ID: | CO000465 |
Collection Summary: | None |
Sample Type: | Blood |
Collection Method: | Lithium-heparin vacutainer tubes |
Storage Conditions: | -80C |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000485 |
Treatment Summary: | No treatment |
Sample Preparation:
Sampleprep ID: | SP000478 |
Sampleprep Summary: | None |
Extraction Method: | Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis. |
Extract Storage: | -80°C |
Sample Derivatization: | No |
Sample Spiking: | 25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate |
Combined analysis:
Analysis ID | AN000705 | AN000706 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | HILIC | HILIC |
Chromatography system | Shimadzu 20AD | Shimadzu 20AD |
Column | Shodex NH2P-40 2E (250× 2.0 mm) | Shodex NH2P-40 2E (250× 2.0 mm) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | ABI Sciex 5500 QTrap | ABI Sciex 5500 QTrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | AUC | AUC |
Chromatography:
Chromatography ID: | CH000510 |
Chromatography Summary: | HILIC |
Instrument Name: | Shimadzu 20AD |
Column Name: | Shodex NH2P-40 2E (250× 2.0 mm) |
Column Pressure: | 920~2600 psi |
Column Temperature: | 25C |
Flow Gradient: | 0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end. |
Flow Rate: | 0.2 ml/min |
Solvent A: | 95% water/5% acetonitrile; 20 mM ammonium carbonate; 38 mM ammonium hydroxide, pH 9.75 |
Solvent B: | 100% acetonitrile |
Analytical Time: | 46.1 min |
Oven Temperature: | 25C |
Sample Loop Size: | 10 ul |
Sample Syringe Size: | 100 ul |
Chromatography Type: | HILIC |
MS:
MS ID: | MS000627 |
Analysis ID: | AN000705 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |
Collision Energy: | Compound-specific |
Collision Gas: | High |
Ion Source Temperature: | 500C |
Ion Spray Voltage: | 5500V |
Ionization: | ESI |
Mass Accuracy: | 0.1Da |
MS ID: | MS000628 |
Analysis ID: | AN000706 |
Instrument Name: | ABI Sciex 5500 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | NEGATIVE |
Collision Energy: | Compound-specific |
Collision Gas: | High |
Ion Source Temperature: | 500C |
Ion Spray Voltage: | -4500V |
Ionization: | ESI |
Mass Accuracy: | 0.1Da |