Return to study ST000460 main page
MB Sample ID: SA023251
Local Sample ID: | AGY571_010 |
Subject ID: | SU000481 |
Subject Type: | Cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Cell Strain Details: | AGY571 |
Subject Comments: | See study design section for cell number per sample for cell counts |
Cell Primary Immortalized: | Immortalized |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000481 |
Subject Type: | Cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Cell Strain Details: | AGY571 |
Subject Comments: | See study design section for cell number per sample for cell counts |
Cell Primary Immortalized: | Immortalized |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
AGY571_010 | SA023251 | FL005649 | Doxycycline | Treatment |
AGY571_010 | SA023251 | FL005649 | 500 ng/mL | Dox Concentration |
AGY571_010 | SA023251 | FL005649 | GFP (ATG7-/-) | Ectopic Gene Expression |
Collection:
Collection ID: | CO000475 |
Collection Summary: | Cells were counted, cells were collected via centrifugation, washed twice with cold 1x PBS, PBS removed and flash frozen. Pellets were saved at -80 C. |
Sample Type: | Cell |
Collection Method: | Pellet |
Collection Location: | Lab |
Collection Frequency: | 48 hours after Dox treatment |
Storage Conditions: | Pellet at -80C |
Storage Vials: | 1.5 mL microfuge tube |
Tissue Cell Identification: | AGY571 cells |
Treatment:
Treatment ID: | TR000495 |
Treatment Summary: | Cells were treated with vehicle (dH2O) or 500 ng/mL doxycycline. |
Treatment Compound: | Doxycycline |
Treatment Dose: | 0.5-1.0 ug/mL |
Treatment Dosevolume: | 5 mL |
Treatment Doseduration: | 48 hours |
Treatment Vehicle: | dH2O |
Cell Growth Container: | 6-well plate |
Sample Preparation:
Sampleprep ID: | SP000488 |
Sampleprep Summary: | Amino acid |
Sampleprep Protocol Filename: | SB_AA_Assay.pdf |
Combined analysis:
Analysis ID | AN000721 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Agilent 1290 Infinity |
Column | Waters AccQ Tag (100 x 2mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ |
Ion Mode | POSITIVE |
Units | nmol/mg protein |
Chromatography:
Chromatography ID: | CH000518 |
Chromatography Summary: | Amino Acid |
Methods Filename: | SB_AA_Assay.pdf |
Instrument Name: | Agilent 1290 Infinity |
Column Name: | Waters AccQ Tag (100 x 2mm,1.7um) |
Column Temperature: | 55 |
Flow Rate: | 0.7 mL/min |
Solvent A: | AccQ·Tag Eluent A |
Solvent B: | accQ·Tag Eluent B |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000641 |
Analysis ID: | AN000721 |
Instrument Name: | Agilent 6490 QQQ |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | Amino Acids |
Ion Mode: | POSITIVE |