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MB Sample ID: SA026178

Local Sample ID:52_9
Subject ID:SU000524
Subject Type:Humans
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Subject:

Subject ID:SU000524
Subject Type:Humans
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
52_9SA026178FL00657052Time(weeks)
52_9SA026178FL006570GLP-1 analogueTreatment

Collection:

Collection ID:CO000518
Collection Summary:The samples analyzed were obtained in a fasting state. Samples were taken at inclusion, after 8 weeks of weight loss (baseline), and after 4 and 52 weeks of weight maintenance.
Collection Protocol ID:ClinicalTrials.gov Identifier: NCT02094183
Sample Type:Blood

Treatment:

Treatment ID:TR000538
Treatment Summary:22 indivuals received 1.2 mg of liraglutide injections (Victoza, Novo Nordisk)daily for 1 year, 20 controls did not receive treatment

Sample Preparation:

Sampleprep ID:SP000531
Sampleprep Summary:Prior to the NMR measurements, 260 μL of EDTA plasma and 260 μL of a sodium phosphate buffer (75 mmol/L Na2HPO4 in 80%/20% H2O/D2O, pH 7.4; including also 0.08% sodium 3-(trimethylsilyl)propionate-2,2,3,3-d4 and 0.04% sodium azide) was mixed and moved to the NMR tubes. All the liquid handling steps for plasma samples were done with a PerkinElmer JANUS Automated Workstation. The NMR metabolomics laboratory setup combined Bruker AVANCE III 500 MHz and Bruker AVANCE III HD 600 MHz spectrometers

Analysis:

MB Sample ID:SA026178
Analysis ID:AN000771
Analysis Type:NMR
Num Factors:8
Num Metabolites:12
Units:mmol/l

NMR:

NMR ID:NM000091
Analysis ID:AN000771
Instrument Name:Bruker Avance III 500 MHz and Bruker AVANCE III HD 600 MHz
Instrument Type:FT-NMR
NMR Experiment Type:1D 1H
Spectrometer Frequency:500 and 600 MHz
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