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MB Sample ID: SA026263

Local Sample ID:ms5459-4
Subject ID:SU000527
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

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Subject:

Subject ID:SU000527
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Species Group:Human

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms5459-4SA026263FL006577treated-10 nM R1881Treatment Factor
ms5459-4SA026263FL00657724 hours13C media incubation time

Collection:

Collection ID:CO000521
Collection Summary:LNCaP cell media
Sample Type:Cultured cells

Treatment:

Treatment ID:TR000541
Treatment Summary:LNCaP cells that were serum starved and incubated with 2mM [U-13C]glutamine and treated with dose response of a synthetic androgen (R1881) for 24 or 72 hours.

Sample Preparation:

Sampleprep ID:SP000534
Sampleprep Summary:Concentration of Amino Acids metabolites in cell media

Combined analysis:

Analysis ID AN000774
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Thermo Quantum Ultra
Ion Mode POSITIVE
Units micromolar

Chromatography:

Chromatography ID:CH000554
Instrument Name:Waters Acquity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000681
Analysis ID:AN000774
Instrument Name:Thermo Quantum Ultra
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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