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MB Sample ID: SA026527
Local Sample ID: | LD10_31 |
Subject ID: | SU000531 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000531 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
LD10_31 | SA026527 | FL006715 | LD10 | time_point |
Collection:
Collection ID: | CO000525 |
Collection Summary: | tissue was harvested, flash frozen in LiN2, milled with LiN2, and weighed on dry ice for metabolomics analysis |
Sample Type: | Liver |
Treatment:
Treatment ID: | TR000545 |
Treatment Summary: | Tissues were harvested at the appropriate time point during or post-pregnancy |
Sample Preparation:
Sampleprep ID: | SP000538 |
Sampleprep Summary: | Frozen and milled liver tissue was suspended to 10 mg/ml in an extraction solution containing methanol/acetonitrile/water at a ratio of 5:3:2 (v/v/v). Suspensions were vortexed at 4C for 30 minutes and centrifuged at 10,000 g for 10 minutes, 4C. Supernatants were isolated and analyzed by LC-MS. |
Combined analysis:
Analysis ID | AN000780 | AN000781 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Kinetex XB-C18 | Kinetex XB-C18 |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | arbitrary units | arbitrary units |
Chromatography:
Chromatography ID: | CH000559 |
Instrument Name: | Thermo Vanquish |
Column Name: | Kinetex XB-C18 |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000687 |
Analysis ID: | AN000780 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS000688 |
Analysis ID: | AN000781 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |