Return to study ST000514 main page
MB Sample ID: SA026750
Local Sample ID: | ms5629-12 |
Subject ID: | SU000536 |
Subject Type: | Mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000536 |
Subject Type: | Mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
ms5629-12 | SA026750 | FL006764 | MIGIRKO | Group |
ms5629-12 | SA026750 | FL006764 | IR -/- IGFR -/- | Genotype |
ms5629-12 | SA026750 | FL006764 | saline | Treatment |
Collection:
Collection ID: | CO000530 |
Collection Summary: | mouse serum |
Sample Type: | Muscle |
Treatment:
Treatment ID: | TR000550 |
Treatment Summary: | To determine which protein degradation pathways downstream of IR and IGF1R contribute to changes in amino acid and mitochondrial metabolite pools, we will treat control, M-IR-/-, MIGIRKO, and HFD obese mice with inhibitors of autophagy or proteasome. We will treat 5 animals each of control, M-IR-/-, MIGIRKO, and HFD mice with vehicle, colchicine to inhibit autophagy, or MG132 to inhibit proteasome activity, then measure amino acid metabolites in serum as well as TCA cycle and amino acid metabolites in muscle tissue. |
Sample Preparation:
Sampleprep ID: | SP000543 |
Sampleprep Summary: | Concentration of amino acid metabolites in serum |
Combined analysis:
Analysis ID | AN000787 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Waters Acquity |
Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantum Ultra |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000564 |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS000694 |
Analysis ID: | AN000787 |
Instrument Name: | Thermo Quantum Ultra |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | POSITIVE |