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MB Sample ID: SA031865
Local Sample ID: | 11-0A |
Subject ID: | SU000612 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 60 |
Gender: | male |
Human Race: | Caucasian |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000612 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 60 |
Gender: | male |
Human Race: | Caucasian |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
11-0A | SA031865 | FL007200 | 0 uL | Plasma Volume Extracted |
Collection:
Collection ID: | CO000606 |
Collection Summary: | Plasma from blood drawn intravenously |
Sample Type: | Blood |
Collection Method: | IV |
Additives: | EDTA |
Blood Serum Or Plasma: | Plasma |
Treatment:
Treatment ID: | TR000626 |
Treatment Summary: | no treatment |
Sample Preparation:
Sampleprep ID: | SP000619 |
Sampleprep Summary: | Protein precipitation and drying followed by derivatization via methoxyamine and MSTFA |
Extraction Method: | Protein precipitation |
Sample Derivatization: | 18 mg/mL methoxyamine hydrochloride in pyridine for 30 min at 50°C, followed by trimethylsilylation with N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA) for 30 min at 50°C |
Sample Spiking: | retention-time-lock internal standard of perdeuterated myristic acid |
Combined analysis:
Analysis ID | AN000904 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 6890N |
Column | Agilent DB5-MS (15m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5975B |
Ion Mode | POSITIVE |
Units | Peak area (Log2 transformed) |
Chromatography:
Chromatography ID: | CH000642 |
Chromatography Summary: | GC/MS methods follow previous studies using a 6890 N GC connected to a 5975B Inert single quadrupole MS (Agilent Technologies, Santa Clara, CA) (Bonikos et al. 1975; Fiehn 2008; Kind et al. 2009). The two wall-coated, open-tubular GC columns connected in series are both from J&W/Agilent (part 122–5512), DB5-MS, 15 meters in length, 0.25 mm in diameter, with an 0.25-l m luminal film. Positive ions generated with conventional electron-ionization at 70 eV are scanned broadly from 600 to 50 m/z in the detector throughout the 45 min cycle time. |
Instrument Name: | Agilent 6890N |
Column Name: | Agilent DB5-MS (15m x 0.25mm,0.25um) |
Flow Rate: | 2.0 mL/min |
Time Program: | initial GC oven temperature of 60°C increased at 10°C/min to a final temperature of 325°C |
Chromatography Type: | GC |
MS:
MS ID: | MS000804 |
Analysis ID: | AN000904 |
Instrument Name: | Agilent 5975B |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |