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MB Sample ID: SA042056
| Local Sample ID: | 03_CBS_3 |
| Subject ID: | SU000789 |
| Subject Type: | plant |
| Subject Species: | Glycine max |
| Taxonomy ID: | 3847 |
| Species Group: | Plant |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000789 |
| Subject Type: | plant |
| Subject Species: | Glycine max |
| Taxonomy ID: | 3847 |
| Species Group: | Plant |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 03_CBS_3 | SA042056 | FL008780 | Cultivar | type |
Collection:
| Collection ID: | CO000783 |
| Collection Summary: | Fifteen wild and 11 cultivated black soybeans were selected from the soybean germplasm collections that have been maintained by the National Institute of Crop Science in Korea |
| Sample Type: | Plant |
Treatment:
| Treatment ID: | TR000803 |
| Treatment Summary: | The planting density was 15 cm between plants in a row, and the rows were 70 cm apart. Field management, including irrigation, fertilizer application, and pest control followed essentially normal agricultural practices according to Rural Development Administration standard protocol |
Sample Preparation:
| Sampleprep ID: | SP000796 |
| Sampleprep Summary: | Dried black soybeans were ground into a fine powder and sieved through a screen with a 55 mesh count, then 40 mg of the powder was mixed with 70% aqueous methanol (4mL, 1% HCl) containing 1.5 μg/ml phlorizin as an internal standard and sonicated for 30 min. The mixture was kept for approximately 16 h at room temperature in the dark.vacuum.Supernatants were filtered through a 0.20-μm syringe filter and freeze dried then reconstituted with 0.5 ml of 50% aqueous methanol for LC-MS analysis. |
Combined analysis:
| Analysis ID | AN001212 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 1290 |
| Column | Prevail C18 |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Bruker Daltonics |
| Ion Mode | POSITIVE |
| Units | peal area |
Chromatography:
| Chromatography ID: | CH000849 |
| Instrument Name: | Agilent 1290 |
| Column Name: | Prevail C18 |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001107 |
| Analysis ID: | AN001212 |
| Instrument Name: | Bruker Daltonics |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
