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MB Sample ID: SA051168
Local Sample ID: | Patient 14 |
Subject ID: | SU000917 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Subject:
Subject ID: | SU000917 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Patient 14 | SA051168 | FL009854 | Negative | Malaria Infection Status |
Collection:
Collection ID: | CO000911 |
Collection Summary: | Breath collection was performed as previously reported with alterations detailed here (see PMID: 25810441). In brief, ≥ 1 L of exhaled breath was collected in a 3 L SamplePro Flexfilm sample bag (SKC Inc.). Using a set flow pump (ACTI-VOC, Markes International), exactly 1 L of breath was pumped through an inert stainless-steel sorbent tube with a triple bed sorbent composed of Tenax 60/80/ Carbograph 1 60/80/ Carboxen 1003 40/60 (Camsco). Sorbent tubes were stored at -20°C prior to shipment on black ice for later mass spectrometric analysis. |
Sample Type: | Breath |
Treatment:
Treatment ID: | TR000931 |
Treatment Summary: | N/A |
Sample Preparation:
Sampleprep ID: | SP000924 |
Sampleprep Summary: | N/A |
Combined analysis:
Analysis ID | AN001440 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus 4D GC |
Column | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | raw abundance |
Chromatography:
Chromatography ID: | CH001011 |
Chromatography Summary: | All samples were run with a TurboMatrix 650 ATD (Perkin Elmer) connected to a Leco Pegasus 4D GCxGC-TOFMS system. Before analysis, sorbent tubes were brought to room temperature and purged for 5 min with BiP N2 (Airgas) at 60 mL/min. A gaseous standard mixture (20.1 ng fluorobenzene, 18.6 ng toluene-D8, 21.7 ng bromofluorobenzene, 20.3 ng 1,2-dichlorobenzene-D4) was added to each tube by the TurboMatrix 650 immediately prior to analysis. Tubes were desorbed at 270°C, 40 mL/min He flow, with recollection on a secondary Tenax cold trap at 10°C. Analytes were released from the secondary trap by heating to 295°C with 20% transferred to the GC/MS. The GC had a 30 m length x 0.25 mm ID x 0.25 µm film thickness DB-5 column (Agilent). The GC oven was programmed to hold at 40°C for 3 min, ramp 5°C/min to 200°C, then ramp 10°C/min to 250°C, final ramp 25°C/min to 300°C, then hold at 300°C for 3 min. The TOFMS had a sampling frequency of 50 Hz and a mass recording range of 34-400 amu. |
Instrument Name: | Leco Pegasus 4D GC |
Column Name: | Agilent DB5-MS (30m × 0.25mm, 0.25um) |
Flow Rate: | 1.2 mL/min |
Chromatography Type: | GC |
MS:
MS ID: | MS001330 |
Analysis ID: | AN001440 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |