Return to study ST000417 main page
MB Sample ID: SA051600
Local Sample ID: | 208_NO2PM_PRE |
Subject ID: | SU000923 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Weight Or Weight Range: | Normal/Overweight/Obese |
Gender: | Male/Female |
Human Race: | Black/White |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000923 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Weight Or Weight Range: | Normal/Overweight/Obese |
Gender: | Male/Female |
Human Race: | Black/White |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
208_NO2PM_PRE | SA051600 | FL009943 | NO2PM | Group |
208_NO2PM_PRE | SA051600 | FL009943 | Pre | Collection Time |
208_NO2PM_PRE | SA051600 | FL009943 | M | Gender |
Collection:
Collection ID: | CO000917 |
Collection Summary: | Plasma collected Pre-exposure, immediately Post-exposure or 24 hour Post Exposure |
Sample Type: | Blood |
Treatment:
Treatment ID: | TR000937 |
Treatment Summary: | Healthy adults were exposed to clean air and subsequently to air into which PM concentrated from the ambient, outdoor environment or PM in combination with NO2 had been introduced. |
Sample Preparation:
Sampleprep ID: | SP000930 |
Sampleprep Summary: | Aliquots of 30µL plasma were mixed with 1mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
Combined analysis:
Analysis ID | AN001448 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Leco Pegasus 4D GC |
Column | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
MS Type | EI |
MS instrument type | GC x GC-TOF |
MS instrument name | Leco Pegasus 4D GCxGC TOF |
Ion Mode | POSITIVE |
Units | Intensity |
Chromatography:
Chromatography ID: | CH001017 |
Instrument Name: | Leco Pegasus 4D GC |
Column Name: | Restek Rtx-5Sil (30m x 0.25mm,0.25um) |
Column Pressure: | 12.8psi |
Flow Gradient: | constant flow |
Flow Rate: | 1ml/min |
Injection Temperature: | 250C |
Retention Index: | FAME markers C8-C30 |
Chromatography Type: | GC |
MS:
MS ID: | MS001338 |
Analysis ID: | AN001448 |
Instrument Name: | Leco Pegasus 4D GCxGC TOF |
Instrument Type: | GC x GC-TOF |
MS Type: | EI |
Ion Mode: | POSITIVE |