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MB Sample ID: SA052972
| Local Sample ID: | Prtcl1_Run2_IDH1wt2 |
| Subject ID: | SU000941 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | 293T cells |
| Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000941 |
| Subject Type: | Animal |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Strain Details: | 293T cells |
| Species Group: | Human |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Prtcl1_Run2_IDH1wt2 | SA052972 | FL010022 | IDH1wt | IDH expression |
Collection:
| Collection ID: | CO000935 |
| Collection Summary: | Cells were washed x3 in cold PBS, then scraped into 80% methanol containing 20 uM L-norvaline |
| Collection Protocol Filename: | GC-MS analysis of D-2-hydroxyglutarate |
| Sample Type: | Adherent cultured mammalian cells |
Treatment:
| Treatment ID: | TR000955 |
| Treatment Summary: | Cells transfected with empty vector or vector expressing wild-type or mutant isocitrate dehydrogenase (IDH1 or IDH2; mutations respectively R132H or R172K) |
Sample Preparation:
| Sampleprep ID: | SP000948 |
| Sampleprep Summary: | Methanol/water extracts were counter-extracted with chloroform to remove non-polar metabolites, dried in a centrifugal evaporator, and derivatized in 60 ul 1:1 mix acetonitrile: MTBSTFA, with heating for 75 min at 75C |
Combined analysis:
| Analysis ID | AN001471 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Shimadzu |
| Column | Rxi-5ms |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Shimadzu QP2010 Plus |
| Ion Mode | POSITIVE |
| Units | ion counts |
Chromatography:
| Chromatography ID: | CH001034 |
| Instrument Name: | Shimadzu |
| Column Name: | Rxi-5ms |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001359 |
| Analysis ID: | AN001471 |
| Instrument Name: | Shimadzu QP2010 Plus |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
