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MB Sample ID: SA053004
Local Sample ID: | Prtcl2_Run4_vec1 |
Subject ID: | SU000941 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | 293T cells |
Species Group: | Human |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000941 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Strain Details: | 293T cells |
Species Group: | Human |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
Prtcl2_Run4_vec1 | SA053004 | FL010025 | vector | IDH expression |
Collection:
Collection ID: | CO000935 |
Collection Summary: | Cells were washed x3 in cold PBS, then scraped into 80% methanol containing 20 uM L-norvaline |
Collection Protocol Filename: | GC-MS analysis of D-2-hydroxyglutarate |
Sample Type: | Adherent cultured mammalian cells |
Treatment:
Treatment ID: | TR000955 |
Treatment Summary: | Cells transfected with empty vector or vector expressing wild-type or mutant isocitrate dehydrogenase (IDH1 or IDH2; mutations respectively R132H or R172K) |
Sample Preparation:
Sampleprep ID: | SP000948 |
Sampleprep Summary: | Methanol/water extracts were counter-extracted with chloroform to remove non-polar metabolites, dried in a centrifugal evaporator, and derivatized in 60 ul 1:1 mix acetonitrile: MTBSTFA, with heating for 75 min at 75C |
Combined analysis:
Analysis ID | AN001471 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Shimadzu |
Column | Rxi-5ms |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Shimadzu QP2010 Plus |
Ion Mode | POSITIVE |
Units | ion counts |
Chromatography:
Chromatography ID: | CH001034 |
Instrument Name: | Shimadzu |
Column Name: | Rxi-5ms |
Chromatography Type: | GC |
MS:
MS ID: | MS001359 |
Analysis ID: | AN001471 |
Instrument Name: | Shimadzu QP2010 Plus |
Instrument Type: | Single quadrupole |
MS Type: | EI |
Ion Mode: | POSITIVE |