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MB Sample ID: SA054699
| Local Sample ID: | MC |
| Subject ID: | SU000960 |
| Subject Type: | Animal |
| Subject Species: | Callinectes sapidus |
| Taxonomy ID: | 6763 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU000960 |
| Subject Type: | Animal |
| Subject Species: | Callinectes sapidus |
| Taxonomy ID: | 6763 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| MC | SA054699 | FL010105 | mud crab diet | Diet |
Collection:
| Collection ID: | CO000954 |
| Collection Summary: | Urine was collected from Blue Crabs |
| Sample Type: | Urine |
| Collection Method: | 22-gauge needle with gentle suction |
| Collection Location: | Skidway Institute of Oceanography |
| Collection Frequency: | every other day |
| Storage Conditions: | -20C |
Treatment:
| Treatment ID: | TR000974 |
| Treatment Summary: | Blue crabs were fed mud crabs or oysters |
Sample Preparation:
| Sampleprep ID: | SP000967 |
| Sampleprep Summary: | Dilute and shoot, urine |
| Processing Method: | mixed 1 (sample): 5 (MeOH), centrifuged, supernatent analyzed |
| Processing Storage Conditions: | on ice |
Combined analysis:
| Analysis ID | AN001511 | AN001512 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001065 |
| Chromatography Summary: | Basic pH HILIC method |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Waters Acquity BEH Amide (75 x 2.1mm,1.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | (time, %A): 0, 5; 3, 63; 7, 63; 7.1, 5; 7.2, 0.5; 9.5, 5; 9.9,5. |
| Flow Rate: | (time, rate (ml/min)): 0, 0.3; 3, 0.3; 7, 0.3; 7.1, 0.3; 7.2, 0.5; 9.5, 0.5; 9.9, 0.3. |
| Sample Injection: | 2 ul |
| Solvent A: | 100% water; 10 mM sodium acetate; 0.05% ammonium hydroxide |
| Solvent B: | 100% acetonitrile; 0.05% ammonium hydroxide |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001394 |
| Analysis ID: | AN001511 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Capillary Temperature: | 275 C |
| Collision Energy: | 10, 30, 50 NCE stepped |
| Collision Gas: | nitrogen |
| Fragmentation Method: | HCD |
| Spray Voltage: | 3.00 kV |
| Automatic Gain Control: | 3.00E+6 |
| Resolution Setting: | 240,000 |
| Scanning Range: | 70-1050 |
| MS ID: | MS001395 |
| Analysis ID: | AN001512 |
| Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Capillary Temperature: | 275 C |
| Collision Energy: | 10, 30, 50 NCE stepped |
| Collision Gas: | nitrogen |
| Fragmentation Method: | HCD |
| Spray Voltage: | 2.80 kV |
| Automatic Gain Control: | 3.00E+6 |
| Resolution Setting: | 240,000 |
| Scanning Range: | 70-1050 |
