Return to study ST000955 main page
MB Sample ID: SA057214
Local Sample ID: | WT1_48h_0mM_2 |
Subject ID: | SU000994 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 OT-1 |
Age Or Age Range: | 6-8 weeks |
Animal Animal Supplier: | Breeding at Moffitt for several generations, originally from Jax |
Animal Light Cycle: | 12 hour |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU000994 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl6 OT-1 |
Age Or Age Range: | 6-8 weeks |
Animal Animal Supplier: | Breeding at Moffitt for several generations, originally from Jax |
Animal Light Cycle: | 12 hour |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
WT1_48h_0mM_2 | SA057214 | FL010312 | 48 | Time activated with peptide |
WT1_48h_0mM_2 | SA057214 | FL010312 | - | DFMO treatment (mM) |
Collection:
Collection ID: | CO000988 |
Collection Summary: | At 48 or 72 hours cells in culture medium were centrifuged on a Ficoll gradient to enrich viable cells. Cells were washed 1x with PBS, counted, and frozen as cell pellets at -80. |
Sample Type: | Cultured cells |
Treatment:
Treatment ID: | TR001008 |
Treatment Summary: | Splenic CD8+ T cells were isolated from 4 OT-1 mice (WT1, 3, 7, 8) by negative immunomagnetic selection. T cells stimulated with ova-derived peptide (SIINFEKL) that binds with high affinity to the OT-1 transgenic TCR and 10ng/mL IL-2 with and without 5mM DFMO for 48-72 hours. Cell stoarge: 37 degrees, Cell growth container: 24 well plate, cell media: RPMI1640 with glutamine. Added components were: NEAA, Sodium Pyruvate, Fetal Bovine Serum, Fungizone, Gentimycin, Pen/strep. |
Sample Preparation:
Sampleprep ID: | SP001001 |
Sampleprep Summary: | None |
Sampleprep Protocol Filename: | GMetabolomics_LCMS_Protocol_092117.pdf Appendix_A_Internal_Standard_Prep_GLCMS.pdf |
Combined analysis:
Analysis ID | AN001566 | AN001567 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | peak height | peak height |
Chromatography:
Chromatography ID: | CH001098 |
Methods Filename: | GMetabolomics_LCMS_Protocol_092117.pdf |
Instrument Name: | Thermo Dionex Ultimate 3000 |
Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
Flow Rate: | 350 ul/min |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001444 |
Analysis ID: | AN001566 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | POSITIVE |
MS ID: | MS001445 |
Analysis ID: | AN001567 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
Ion Mode: | NEGATIVE |