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MB Sample ID: SA061678

Local Sample ID:ms6722-23
Subject ID:SU001035
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Subject:

Subject ID:SU001035
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
ms6722-23SA061678FL010679DCGrouping

Collection:

Collection ID:CO001029
Collection Summary:After obtaining informed consent, portal and peripheral venous blood (4 ml of each) and bile (2 mL) was collected intraoperatively in a red-top tube by a Mayo Clinic LT surgeon. Blood was placed on ice, promptly fractionated by centrifugation, divided into 100 μL aliquots, and stored at -80°C.
Sample Type:Blood (serum)

Treatment:

Treatment ID:TR001049
Treatment Summary: We prospectively enrolled three groups of participants from the Mayo Clinic Liver Transplant inpatient service and outpatient clinics and have collected samples from: i) 9 patients with PSC who underwent living- donor LT, ii) 15 donors (normal controls), and iii) 20 patients with cirrhosis due to a disorder other than PSC who underwent LT (disease controls). The following inclusion and exclusion criteria were applied: Inclusion criteria 1. Adult (age>18 years). 2. PSC patient undergoing LT, healthy living donor, or other chronic liver disease patient undergoing LT. Exclusion Criteria: 1. Females who are pregnant or attempting to become pregnant. 2. Concomitant liver disease (e.g. chronic viral hepatitis in addition to PSC). 3. Acute intestinal disease (infectious enterocolitis, IBD flare) in the past 6 months. 4. Treatment with any investigational drugs within the past 6 months. 5. Use of antibiotics within the past 4 weeks. 6. Any previous organ transplant. 7. Hemodialysis.

Sample Preparation:

Sampleprep ID:SP001042
Sampleprep Summary:nefa acid concentrations

Combined analysis:

Analysis ID AN001625
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6460 QQQ
Ion Mode NEGATIVE
Units uM

Chromatography:

Chromatography ID:CH001143
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH C18 (150 x 2.1mm,1.7um)
Chromatography Type:Reversed phase

MS:

MS ID:MS001501
Analysis ID:AN001625
Instrument Name:Agilent 6460 QQQ
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:uM
Ion Mode:NEGATIVE
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