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MB Sample ID: SA069385
Local Sample ID: | R3 |
Subject ID: | SU001076 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | NA |
Age Or Age Range: | NA |
Gender: | Male |
Human Race: | NA |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001076 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | NA |
Age Or Age Range: | NA |
Gender: | Male |
Human Race: | NA |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
R3 | SA069385 | FL011115 | Reagent Blank | Description |
Collection:
Collection ID: | CO001070 |
Collection Summary: | Purchased Serum from Sigma Aldrich. |
Sample Type: | Human Serum from Commercial Source |
Collection Method: | NA |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001090 |
Treatment Summary: | None. |
Sample Preparation:
Sampleprep ID: | SP001083 |
Sampleprep Summary: | Serum samples (30 µL) were subjected to sequential solvent extraction once each with 1 mL of acetonitrile: isopropanol: water (3:3:2, v/v) ratio and 500 µL of acetonitrile: water (1:1, v/v) ratio mixtures at 4 C.22 Adonitol and d4-succinic acid (both 5 µL from 10 mg/ml stock) were added to each aliquots as two internal standards prior to the extraction. The pooled extracts (~ 1500 µL) from the two steps were dried under vacuum at 4 C prior to chemical derivatization. Dummy extractions performed on blank tubes served as extraction blanks to account for background (extraction solvent, derivatization reagents) noise and other sources of contamination (septa, liner, column, vials, handling etc.). Blanks were only used to see that no carry overs occurred during randomized run orders and to manually filter out contaminating chemicals from the combined list of features. Samples were then sequentially derivatized with methoxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) as described elsewhere.23,24 Steps involved addition of 10 μL of MeOX (20 mg/mL) in pyridine incubated under shaking at 55 °C for 60 min followed by trimethylsilylation at 60 °C for 60 min after adding 90 μL MSTFA. |
Sampleprep Protocol ID: | Fiehn et al., 2008 |
Sampleprep Protocol Comments: | NA |
Processing Method: | Fiehn et al., 2008 |
Processing Storage Conditions: | On ice |
Extraction Method: | Fiehn et al., 2008 |
Extract Enrichment: | None |
Extract Cleanup: | None |
Sample Resuspension: | NA |
Sample Derivatization: | Methoxyaminatin + silylation (MSTFA) |
Sample Spiking: | Adonitol |
Subcellular Location: | NA |
Combined analysis:
Analysis ID | AN001698 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | QEOrbitrap-GC-MS |
Column | TraceGOLD TG-5SILMS |
MS Type | EI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE |
Units | Abundance |
Chromatography:
Chromatography ID: | CH001197 |
Chromatography Summary: | A robotic arm TriPlus™ RSH autosampler (Thermo Scientific™, Bremen, Germany) injected 1µL of derivatized sample into a split/splitless (SSL) injector at 250 °C using a 1:100 split flow on TRACE™ 1310 gas chromatograph (Thermo Scientific™, Austin, TX). Helium carrier gas at a flow rate of 1 mL/min was used for separation on a Thermo Scientific™ TraceGOLD™ TG-5SILMS 30 m length × 0.25 mm i.d. × 0.25 µm film thickness column. The initial oven temperature was held at 70 °C for 4 min, followed by an initial gradient of 20 °C/min ramp rate. The final temperature was 320 °C and held for 8 min. |
Instrument Name: | QEOrbitrap-GC-MS |
Column Name: | TraceGOLD TG-5SILMS |
Chromatography Type: | GC |
MS:
MS ID: | MS001573 |
Analysis ID: | AN001698 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | EI |
Ion Mode: | POSITIVE |
Fragmentation Method: | EI |
Helium Flow: | 1 ml/min |
Ion Source Temperature: | 250 |