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MB Sample ID: SA071586
| Local Sample ID: | WT_TCP_48H_202 |
| Subject ID: | SU001097 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild Type C57BL/6 and CAR Knockout |
| Age Or Age Range: | Approximately 8 weeks old |
| Gender: | Male |
| Animal Light Cycle: | 12 h |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001097 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Wild Type C57BL/6 and CAR Knockout |
| Age Or Age Range: | Approximately 8 weeks old |
| Gender: | Male |
| Animal Light Cycle: | 12 h |
| Animal Feed: | ad libitum |
| Animal Water: | ad libitum |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WT_TCP_48H_202 | SA071586 | FL011221 | WT | Genotype |
| WT_TCP_48H_202 | SA071586 | FL011221 | 48 | Time Point (h) |
| WT_TCP_48H_202 | SA071586 | FL011221 | 2 | TCPOBOP (mg/kg) |
Collection:
| Collection ID: | CO001091 |
| Collection Summary: | Liver tissue was snap-frozen in liquid nitrogen and stored at -80C. |
| Sample Type: | Liver |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001111 |
| Treatment Summary: | Each mouse was treated with either a single dose of 2 mg/kg of TCPOBOP or the vehicle control via intraperitoneal injection for either 48 or 72h. |
Sample Preparation:
| Sampleprep ID: | SP001104 |
| Sampleprep Summary: | Liver tissue was homogenized in a methanol-water mixture, dried and resuspended in phosphate buffer containing TSP-d4. |
| Sampleprep Protocol Filename: | GCMS_FattyAcid_protocol.pdf |
| Processing Method: | Homogenization |
Combined analysis:
| Analysis ID | AN001724 |
|---|---|
| Analysis type | MS |
| Chromatography type | GC |
| Chromatography system | Agilent 7890A |
| Column | Agilent HP-5MS |
| MS Type | EI |
| MS instrument type | Single quadrupole |
| MS instrument name | Agilent 5975C |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001218 |
| Instrument Name: | Agilent 7890A |
| Column Name: | Agilent HP-5MS |
| Chromatography Type: | GC |
MS:
| MS ID: | MS001594 |
| Analysis ID: | AN001724 |
| Instrument Name: | Agilent 5975C |
| Instrument Type: | Single quadrupole |
| MS Type: | EI |
| Ion Mode: | POSITIVE |
