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MB Sample ID: SA073570
| Local Sample ID: | 25 |
| Subject ID: | SU001133 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | B6Ly5.2 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001133 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | B6Ly5.2 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 25 | SA073570 | FL011423 | Allogenic | BMT Type |
| 25 | SA073570 | FL011423 | NLRP6KO | Mice type |
| 25 | SA073570 | FL011423 | Small Intestine lumen | Site |
Collection:
| Collection ID: | CO001127 |
| Collection Summary: | Stool lumen contents |
| Sample Type: | Intestinal lumen contents |
Treatment:
| Treatment ID: | TR001147 |
| Treatment Summary: | BMT from naive [B6Ly5.2→B6] and allogenic e allogeneic [B6Ly5.2→Nlrp6-/-] animals |
Sample Preparation:
| Sampleprep ID: | SP001140 |
| Sampleprep Summary: | To determine targeted taurine quantitation, samples (int estinal fecal content) from mice 21d post-transplant were harvested, homogenized, and snap-frozen in liquid N2. Fecal content was we ighed at necropsy. Samples were extracted with acetonitrile spiked with stable isotope-labeled C13 taurine standards. The supernatan t was analyzed by Agilent 6490 mass spectrometer in a positive electrospray ionization mode after liquid chromatography using a HILI C mode and isocratic gradient. Quantitation was performed by calibration to internal standards and expressed in mM after normalizati on for weights. |
Combined analysis:
| Analysis ID | AN001774 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 Infinity |
| Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | Agilent 6410 QQQ |
| Ion Mode | POSITIVE |
| Units | M/100mg stool |
Chromatography:
| Chromatography ID: | CH001254 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001640 |
| Analysis ID: | AN001774 |
| Instrument Name: | Agilent 6410 QQQ |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
