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MB Sample ID: SA075275
| Local Sample ID: | 802_LV |
| Subject ID: | SU001152 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Age Or Age Range: | Within 6 hours of birth |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001152 |
| Subject Type: | Mammal |
| Subject Species: | Ovis aries |
| Taxonomy ID: | 9940 |
| Age Or Age Range: | Within 6 hours of birth |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| 802_LV | SA075275 | FL011646 | Cortisol | Treatment |
| 802_LV | SA075275 | FL011646 | Left_ventricle | Heart Area |
Collection:
| Collection ID: | CO001146 |
| Collection Summary: | Immediately following birth, the lambs were euthanized with an overdose of Euthasol (pentobarbital sodium and phenytoin sodium; Virbac AH Inc), and heart tissue was collected from the right ventricle (RV), left ventricle (LV), and intraventricular septum (IVS) and immediately frozen in liquid nitrogen. Tissue samples were stored at -80 °C until data collection. |
| Sample Type: | Cardiac tissue |
| Collection Method: | Heart tissue was collected under sterile conditions and immediately frozen in liquid nitrogen |
| Collection Location: | University of Florida |
| Storage Conditions: | -80℃ |
| Collection Vials: | Cryovials |
| Storage Vials: | Cryovials |
Treatment:
| Treatment ID: | TR001166 |
| Treatment Summary: | The treatment protocol for this study was previous published in Antolic, A., et al. (2018). Chronic maternal hypercortisolemia in late gestation alters fetal cardiac function at birth. Am J Physiol Regul Integr Comp Physiol 314(3): R342-R352. |
| Treatment Compound: | Hydrocortisone sodium succinate in sodium phosphate (Solu-Cortef; Pfizer, New York, NY, USA) |
| Treatment Dose: | 1 mg/kg/day |
Sample Preparation:
| Sampleprep ID: | SP001159 |
| Sampleprep Summary: | Heart tissue (20-50 mg) was cut and weighed on dry ice before being placed on a sterile agar plate. Thirty μL of deuterium oxide (D2O) was added to each tissue sample before being placed in a 4 mm HR-MAS rotor (Bruker Biospin, Billerica, MA, USA) and leftover D2O (from original 30 μL) was added to the rotor with a pipette after placement of tissue. The samples were kept on dry ice until data acquisition. |
| Sampleprep Protocol Comments: | Organ: Heart |
Analysis:
| MB Sample ID: | SA075275 |
| Analysis ID: | AN001801 |
| Laboratory Name: | Advanced Magnetic Resonance Imaging and Spectroscopy Facility-University of Florida |
| Analysis Type: | NMR |
| Analysis Protocol File: | Hearts_CORT_raw_data_noesy.zip |
| Acquisition Parameters File: | Hearts_CORT_raw_data_noesy.zip |
| Software Version: | Topspin 3.6 |
| Operator Name: | Jacquelyn Walejko |
| Processing Parameters File: | Hearts_CORT_raw_data_noesy.zip |
| Num Factors: | 6 |
| Num Metabolites: | 27 |
| Units: | Area under the curve |
NMR:
| NMR ID: | NM000137 |
| Analysis ID: | AN001801 |
| Instrument Name: | Bruker Avance III |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D 1H |
| Field Frequency Lock: | H2O+D2O |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 4mm HR-MAS |
| NMR Solvent: | D2O |
| NMR Tube Size: | 4mm HRMAS rotor |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | Presaturation |
| Power Level: | 18 W |
| Receiver Gain: | 40.3 |
| Offset Frequency: | 2819.74 |
| Presaturation Power Level: | 3.98E-05 |
| Temperature: | 4C |
| Number Of Scans: | 128 |
| Dummy Scans: | 8 |
| Acquisition Time: | 1.3598 sec |
| Relaxation Delay: | 2 sec |
| Spectral Width: | 10.0155 ppm |
| Num Data Points Acquired: | 16344 |
| Real Data Points: | 32768 |
| Line Broadening: | 2 |
