Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA075896

Local Sample ID:	M-GroupIV_48
Subject ID:	SU001157
Subject Type:	Fish
Subject Species:	Megalobrama amblycephala
Taxonomy ID:	75352

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Subject:

Subject ID:	SU001157
Subject Type:	Fish
Subject Species:	Megalobrama amblycephala
Taxonomy ID:	75352

Factors:

Local Sample ID	MB Sample ID	Factor Level ID	Level Value	Factor Name
M-GroupIV_48	SA075896	FL011669	2	age(years)
M-GroupIV_48	SA075896	FL011669	Yes	mature
M-GroupIV_48	SA075896	FL011669	Yes	spawned
M-GroupIV_48	SA075896	FL011669	Yes	hormone treatment

Collection:

Collection ID:	CO001151
Collection Summary:	The gonads of fish were collected to identify its sex of 1-year-old fish by traditional histological analysis and the muscle samples were only selected from the females (group Ⅰ). As to 2-year-old M. amblycephala, the muscle samples were firstly collected from individuals, and then the gonads were collected to do the histological analysis. Individuals with ovary development before stage Ⅴ were recognized as non-mature females (group Ⅱ), while individuals with ovary development at stage Ⅴdwere recognized as mature females (group ⅡI). For group IV, the individuals which normally spawned during artificial reproduction, were put back to tank and then muscle samples were collected after 24 hours. For each group, muscle samples from 10 individuals were collected. After the collection, muscle samples were frozen in liquid nitrogen immediately and then transferred them into -80℃ for further study.
Sample Type:	Muscle and Gonad tissue

Treatment:

Treatment ID:	TR001171
Treatment Summary:	Samples were divided into four groups: 1-year-old immature M. amblycephala females (group Ⅰ), 2-year-old immature M. amblycephala females (group Ⅰ), 2-year-old mature females before the injection of hormones (group Ⅲ) and 2-year-old females after 24 hours of their successfully spawning by injecting oxytocin (group ⅣanThe artificial oxytocin implementation procedure was as follows. Firstly, the fish were intraperitoneally injected with a luteinizing hormone-releasing hormone from the basal part of the pectoral fin (LRH-A2) (1 µg/kg fish body weight). After 10 h, the second injection was given (0.5 ml/kg body weight) (each ml contained LRH-A2 4 µg, human chorionic gonadotropin (HCG) 500 U, and domperidone (DOM) 5 mg of solution).

Treatment ID:

TR001171

Treatment Summary:

Samples were divided into four groups: 1-year-old immature M. amblycephala females (group Ⅰ), 2-year-old immature M. amblycephala females (group Ⅰ), 2-year-old mature females before the injection of hormones (group Ⅲ) and 2-year-old females after 24 hours of their successfully spawning by injecting oxytocin (group ⅣanThe artificial oxytocin implementation procedure was as follows. Firstly, the fish were intraperitoneally injected with a luteinizing hormone-releasing hormone from the basal part of the pectoral fin (LRH-A2) (1 µg/kg fish body weight). After 10 h, the second injection was given (0.5 ml/kg body weight) (each ml contained LRH-A2 4 µg, human chorionic gonadotropin (HCG) 500 U, and domperidone (DOM) 5 mg of solution).

Sample Preparation:

Sampleprep ID:	SP001164
Sampleprep Summary:	Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.

Sampleprep ID:

SP001164

Sampleprep Summary:

Muscle tissues about 25 mg from each individual were homogenized in 800 µL cold methanol and water (v/v = 1:1) using a Qiagen Tissue-Lyser (Retsch GmBH, Germany). 400 µL supernatant was collected after centrifugation (25000 g, 4 °C, 10 min). The supernatant in the eppendorf tube was removed and the sediment in the tube was dried. Homogenized the sediment again in 800 µL cold dichloromethane and methanol (v/v = 3:1), after centrifuging and then collected 200 µL supernatant for QC (quality control) sample (25000 g, 4 °C, 10 min). The supernatant was evacuated under vacuum, re-collected 100µL supernatant after centrifugation (25000 g, 4 °C, 10 min) for subsequent detection.

Combined analysis:

Analysis ID	AN001807	AN001808
Analysis type	MS	MS
Chromatography type	Unspecified	Unspecified
Chromatography system	Waters	Waters
Column	ion information	ion information
MS Type	ESI	ESI
MS instrument type	QTOF	QTOF
MS instrument name	Waters Synapt G2 XS QTOF	Waters Synapt G2 XS QTOF
Ion Mode	POSITIVE	NEGATIVE
Units	none	none

Chromatography:

Chromatography ID:	CH001273
Instrument Name:	Waters
Column Name:	ion information
Chromatography Type:	Unspecified

MS:

MS ID:	MS001665
Analysis ID:	AN001807
Instrument Name:	Waters Synapt G2 XS QTOF
Instrument Type:	QTOF
MS Type:	ESI
Ion Mode:	POSITIVE

MS ID:	MS001666
Analysis ID:	AN001808
Instrument Name:	Waters Synapt G2 XS QTOF
Instrument Type:	QTOF
MS Type:	ESI
Ion Mode:	NEGATIVE