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MB Sample ID: SA075904
Local Sample ID: | R13 |
Subject ID: | SU001158 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Woman |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001158 |
Subject Type: | Animal |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Woman |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
R13 | SA075904 | FL011670 | OC | Study Group |
Collection:
Collection ID: | CO001152 |
Collection Summary: | The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method. |
Sample Type: | Stem cells culture media |
Treatment:
Treatment ID: | TR001172 |
Treatment Summary: | Culture media samples were collected by the end of each passage, kept under -20C until the moment of analysis. |
Sample Preparation:
Sampleprep ID: | SP001165 |
Sampleprep Summary: | The endometrial mesenchymal stem cells (EnMSC) were collected from menstrual shedding, isolated and cultured in vitro from donors who used or not oral contraceptives. The EnMSC were grown up until the second passage (P2) and 24h before the end of each passage (P0, P1 and P2) the culture medium was completely replaced for the collection. The samples were centrifuged at 400 × g for 5 minutes, filtered with 0,2 µm filter and stored at -80 ºC until the metabolomics analysis without any extraction method. |
Combined analysis:
Analysis ID | AN001809 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | None |
Column | none |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | ABI Sciex 4000 QTrap |
Ion Mode | UNSPECIFIED |
Units | umol |
Chromatography:
Chromatography ID: | CH001274 |
Instrument Name: | None |
Column Name: | none |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS001667 |
Analysis ID: | AN001809 |
Instrument Name: | ABI Sciex 4000 QTrap |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
Ion Mode: | UNSPECIFIED |