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MB Sample ID: SA081502
| Local Sample ID: | e0117p01t01 |
| Subject ID: | SU001239 |
| Subject Type: | Synthetic |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001239 |
| Subject Type: | Synthetic |
| Subject Species: | Escherichia coli |
| Taxonomy ID: | 562 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| e0117p01t01 | SA081502 | FL012424 | standard | Sample Type |
Collection:
| Collection ID: | CO001233 |
| Collection Summary: | Extensive details of the collection parameters are provided in metadata .mat files (see in raw data zip file : README.pdf). Briefly, a Solarix 7T was used to collect FTICR MS data of thousands of MALDI spots comprised of synthetic metabolite mixtures. |
| Collection Protocol ID: | NA |
| Collection Protocol Filename: | There are multiple provided, e.g. please see : raw data zip file, e0114p03t02_instrument_settings.mat |
| Sample Type: | Synthetic Mixture |
| Collection Method: | FTICR MS |
| Collection Location: | Brown University |
| Collection Frequency: | 2000 |
| Collection Duration: | shot_count: 500, 4x averaging, 1 second duration |
| Volumeoramount Collected: | 0.1 nL |
| Storage Conditions: | Room temperature |
| Collection Vials: | 1 |
| Storage Vials: | 36 |
| Collection Tube Temp: | RT |
| Additives: | Aminoacridine matrix |
Treatment:
| Treatment ID: | TR001254 |
| Treatment Summary: | Combinatorial mixtures of metabolites were deposited using a Labcyte Echo 550 on to a MALDI steel plate at 30-60 nL volumes. Aminoacridine matrix was added in the ratio 0.7:1 to 1:.7 to metabolite concentrations. The spot droplets were air dried. No further treatments were performed prior to data acquisition. |
Sample Preparation:
| Sampleprep ID: | SP001247 |
| Sampleprep Summary: | Combinatorial mixtures of metabolites were deposited using a Labcyte Echo 550 on to a MALDI steel plate at 30-60 nL volumes. Aminoacridine matrix was added in the ratio 0.7:1 to 1:.7 to metabolite concentrations. The spot droplets were air dried. No further preparations were performed prior to data acquisition. |
Combined analysis:
| Analysis ID | AN001948 |
|---|---|
| Analysis type | MS |
| Chromatography type | None (Direct infusion) |
| Chromatography system | none |
| Column | none |
| MS Type | MALDI |
| MS instrument type | FT-ICR |
| MS instrument name | Bruker Solarix FT-ICR-MS |
| Ion Mode | POSITIVE |
| Units | mM |
Chromatography:
| Chromatography ID: | CH001413 |
| Instrument Name: | none |
| Column Name: | none |
| Chromatography Type: | None (Direct infusion) |
MS:
| MS ID: | MS001804 |
| Analysis ID: | AN001948 |
| Instrument Name: | Bruker Solarix FT-ICR-MS |
| Instrument Type: | FT-ICR |
| MS Type: | MALDI |
| MS Comments: | Extensive details of the data processing are provided in the readme.pdf file, and scripts for directly reading the data in Python are also provided. |
| Ion Mode: | POSITIVE |
