Return to study ST001184 main page
MB Sample ID: SA082415
Local Sample ID: | 161114_Smp_8102_F |
Subject ID: | SU001252 |
Subject Type: | Other |
Subject Species: | Marine Plankton |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001252 |
Subject Type: | Other |
Subject Species: | Marine Plankton |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
161114_Smp_8102_F | SA082415 | FL012487 | F | Replicate |
Collection:
Collection ID: | CO001246 |
Collection Summary: | Cultured marine plankton cells grown to mid-to-late exponential phase were filtered onto 0.2-micron filters and extracted for metabolites as described in methods. Environmental plankton samples were collected onto 0.2-micron filters during a diel study (Diel_study_StationCastNumber) in the North Pacific. |
Sample Type: | Plankton cells |
Treatment:
Treatment ID: | TR001267 |
Treatment Summary: | Marine plankton cells were cultured according to standard protocols, and environmental samples were collected in situ. |
Sample Preparation:
Sampleprep ID: | SP001260 |
Sampleprep Summary: | Marine plankton cells were extracted using the described protocols. |
Sampleprep Protocol Filename: | SP_Ingalls_extraction_protocol.pdf |
Combined analysis:
Analysis ID | AN001970 | AN001971 | AN001972 | AN001973 | AN001974 |
---|---|---|---|---|---|
Analysis type | MS | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | HILIC | HILIC | HILIC |
Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
Column | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
MS Type | ESI | ESI | ESI | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Waters Xevo TQ-S | Waters Xevo TQ-S | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
Ion Mode | NEGATIVE | NEGATIVE | NEGATIVE | NEGATIVE | POSITIVE |
Units | nanomolar in extraction vial | picomoles per liter of filtered seawater | peak area per liter of filtered seawater | peak area | Peak area |
Chromatography:
Chromatography ID: | CH001425 |
Methods Filename: | CH_Ingalls_LC_protocol.pdf |
Instrument Name: | Waters Acquity I-Class |
Column Name: | SeQuant ZIC-pHILIC (150 x 2.1mm,5um) |
Column Temperature: | 30 |
Flow Rate: | 0.15 mL/min |
Solvent A: | 85% acetonitrile/15% water; 10 mM ammonium carbonate |
Solvent B: | 40% acetonitrile/60% water; 10 mM ammonium carbonate |
Chromatography Type: | HILIC |
MS:
MS ID: | MS001824 |
Analysis ID: | AN001970 |
Instrument Name: | Waters Xevo TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_Ingalls_MS_protocol.pdf |
MS ID: | MS001825 |
Analysis ID: | AN001971 |
Instrument Name: | Waters Xevo TQ-S |
Instrument Type: | Triple quadrupole |
MS Type: | ESI |
MS Comments: | See protocol. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_Ingalls_MS_protocol.pdf |
MS ID: | MS001826 |
Analysis ID: | AN001972 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_Ingalls_MS_protocol.pdf |
MS ID: | MS001827 |
Analysis ID: | AN001973 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol. |
Ion Mode: | NEGATIVE |
Analysis Protocol File: | MS_Ingalls_MS_protocol.pdf |
MS ID: | MS001828 |
Analysis ID: | AN001974 |
Instrument Name: | Thermo Q Exactive HF hybrid Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | See protocol. |
Ion Mode: | POSITIVE |
Analysis Protocol File: | MS_Ingalls_MS_protocol.pdf |