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MB Sample ID: SA082483
| Local Sample ID: | QC-4 |
| Subject ID: | SU001254 |
| Subject Type: | Insect |
| Subject Species: | Apis mellifera |
| Taxonomy ID: | 7460 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001254 |
| Subject Type: | Insect |
| Subject Species: | Apis mellifera |
| Taxonomy ID: | 7460 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| QC-4 | SA082483 | FL012544 | Pool | Treatment |
Collection:
| Collection ID: | CO001248 |
| Collection Summary: | We sampled three bees from 13 cages after four days of continuous exposure to the treatments and immediately placed the samples on dry ice, followed by long-term storage at -80 °C. |
| Sample Type: | Insect tissue |
| Storage Conditions: | Described in summary |
Treatment:
| Treatment ID: | TR001269 |
| Treatment Summary: | Once the bees had an established microbiome, we prepared treatment feeding solutions of 50% sucrose (as a no metal/metalloid control), 50% sucrose spiked with 0.6 mg/L sodium selenate or 50% sucrose with 0.24 mg/L cadmium chloride (Alfa Aesar, Ward Hill, MA) and pollen patties spiked with either 6.0 mg/L selenium or 0.46 mg/L cadmium and allowed the bees to feed ad libitium. |
Sample Preparation:
| Sampleprep ID: | SP001262 |
| Sampleprep Summary: | Samples were freeze-dried, homogenized, and extracted with 30:30:20:20 acetonitrile:methanol:water:isopropanol. Samples were then sonicated, vortexed, and centrifuged before analysis. |
Combined analysis:
| Analysis ID | AN001979 |
|---|---|
| Analysis type | MS |
| Chromatography type | Normal phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF |
| Ion Mode | POSITIVE |
| Units | peak area |
Chromatography:
| Chromatography ID: | CH001428 |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0 min, 1% B; 1 min, 1% B; 8 min, 40% B; 24 min, 100% B; 26.5 min, 100% B; 27 min, 1% B |
| Flow Rate: | 250 ul/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Normal phase |
MS:
| MS ID: | MS001833 |
| Analysis ID: | AN001979 |
| Instrument Name: | Waters Synapt G2 Si QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | We processed the metabolite data (peak picking, alignment, deconvolution, integration, normalization, and spectral matching) with Progenesis Qi software (Nonlinear Dynamics, Durham, NC). |
| Ion Mode: | POSITIVE |
