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MB Sample ID: SA082591
Local Sample ID: | B_cc_1 |
Subject ID: | SU001256 |
Subject Type: | Fungi |
Subject Species: | Ophiocordyceps sinensis;Cordyceps militaris |
Taxonomy ID: | 72228;73501 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001256 |
Subject Type: | Fungi |
Subject Species: | Ophiocordyceps sinensis;Cordyceps militaris |
Taxonomy ID: | 72228;73501 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
B_cc_1 | SA082591 | FL012558 | Ophiocordyceps sinensis | Species |
B_cc_1 | SA082591 | FL012558 | 50% ethanol-soaked | Treatment |
Collection:
Collection ID: | CO001250 |
Collection Summary: | two species were collected: Ophiocordyceps sinensis and Cordyceps militaris |
Sample Type: | fungi |
Treatment:
Treatment ID: | TR001271 |
Treatment Summary: | two treatments: boiled water and 50% ethanol-soaked |
Sample Preparation:
Sampleprep ID: | SP001264 |
Sampleprep Summary: | The extracts were centrifuged (15 min, 13,000 rpm) at 4oC and 3 mL of supernatant was collected. The supernatant was frozen at -80oC and then dried using a vacuum centrifugal evaporator (CHRiST, Alpha 2-4/LSC, Germany). The dried residues were separately dissolved in 3 mL of distilled water. A 600 µL extract was filtered (Millipore Amicon® ULTRA 3 kDa) and filtrates were collected. 3-(Trimethylsilyl) propanesulfonic acid (50 µL) pre-dissolved in D2O (an internal standard) was subsequently added to 450 µL of the filtrates. Finally, aliquots (480 µL) of each extract were transferred to a 5 mm NMR tube (Norell, Morganton, NC, USA) for NMR analysis. |
Analysis:
MB Sample ID: | SA082591 |
Analysis ID: | AN001981 |
Analysis Type: | NMR |
Num Factors: | 4 |
Num Metabolites: | 52 |
Units: | ppm |
NMR:
NMR ID: | NM000150 |
Analysis ID: | AN001981 |
Instrument Name: | Bruker AV III 600 MHz NMR spectrometer |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 MHz |