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MB Sample ID: SA082721
| Local Sample ID: | NEG S-3 |
| Subject ID: | SU001258 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001258 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| NEG S-3 | SA082721 | FL012572 | Sample | Treatment |
Collection:
| Collection ID: | CO001252 |
| Collection Summary: | Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample |
| Sample Type: | Tumor cells |
Treatment:
| Treatment ID: | TR001273 |
| Treatment Summary: | After cells growth in 37°C for 12 h, 0.25mg/mL ginsenosides was fed of each well and continue cultured with for 12h |
Sample Preparation:
| Sampleprep ID: | SP001266 |
| Sampleprep Summary: | Total metabolites was extracted from the per group cell (~1x10e7) using 1 ml of methanolic acetonitrile aqueous solution (2:2:1, v/v), vortex for 60 s, hypothermia for 30 min, 2 times, place the protein for 1 h at -20 ° C, centrifuge at 14000 rcf for 20 min at 4 ° C, and freeze the supernatant. -80 ° C to save the sample |
Combined analysis:
| Analysis ID | AN001983 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1290 |
| Column | |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | ABI Sciex 5600 TripleTOF |
| Ion Mode | NEGATIVE |
| Units | intensity |
Chromatography:
| Chromatography ID: | CH001431 |
| Instrument Name: | Agilent 1290 |
| Column Temperature: | 25 ° C |
| Flow Rate: | 0.3 mL/min |
| Solvent A: | 100% water; 25 mM ammonium acetate |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS001836 |
| Analysis ID: | AN001983 |
| Instrument Name: | ABI Sciex 5600 TripleTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | The ESI source conditions after HILIC chromatographic separation are as follows: Ion Source Gas1 (Gas1): 60, Ion Source Gas 2 (Gas 2): 60, Curtain gas (CUR): 30, source temperature: 600 ° C, Ion Sapary Voltage Floating (ISVF) ± 5500 V (Positive and negative modes); TOF MS scan m/z range: 60-1000 Da, product ion scan m/z range: 25-1000 Da, TOF MS scan accumulation time 0.20 s/spectra, product ion scan accumulation time 0.05 s/spectra; secondary mass spectrometry is obtained using information dependent acquisition (IDA), and uses high sensitivity mode, Declustering potential (DP): ±60 V (both positive and negative modes), Collision Energy: 35±15 eV, IDA settings are as follows Exclude isotopes within 4 Da, Candidate ions to monitor per cycle: 6 |
| Ion Mode: | NEGATIVE |
