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MB Sample ID: SA086379
| Local Sample ID: | EL-NYMC-141-Run 1 |
| Subject ID: | SU001290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001290 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| EL-NYMC-141-Run 1 | SA086379 | FL012866 | Early Disseminated Lyme | Sample Type |
Collection:
| Collection ID: | CO001284 |
| Collection Summary: | Sera from early Lyme disease patients were collected pretreatment at the initial visit to the clinic. Healthy control serum donors were from endemic and non-endemic regions for Lyme disease. |
| Sample Type: | Blood (serum) |
Treatment:
| Treatment ID: | TR001305 |
| Treatment Summary: | N/A |
Sample Preparation:
| Sampleprep ID: | SP001298 |
| Sampleprep Summary: | Small molecule metabolites were extracted from sera and analyzed by LC-MS as previously described. Specifically, cold methanol (60 µl) was added to 20 µl of sera, vortexed and incubated at -20°C for 1 h. Samples were warmed to room temperature for ten minutes, vortexed and centrifuged at 18,000 x g for 30 min. The supernatant (65 µl) was transferred to a new microcentrifuge tube and dried under vacuum. The dried metabolite extract was suspended in 50% methanol (40 µl) centrifuged at 18,000 x g for 15 min. An aliquot (35 µl) was transferred to an autosampler vial for LC-MS analysis. |
| Sampleprep Protocol Comments: | doi:10.1038/nprot.2011.335 |
Combined analysis:
| Analysis ID | AN002036 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Agilent 6520 |
| Column | Poroshell 120 EC-C8 (100 x 2.1mm,2.5um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Agilent 6520 QTOF |
| Ion Mode | POSITIVE |
| Units | Peak Area |
Chromatography:
| Chromatography ID: | CH001476 |
| Instrument Name: | Agilent 6520 |
| Column Name: | Poroshell 120 EC-C8 (100 x 2.1mm,2.5um) |
| Column Temperature: | 50 |
| Flow Gradient: | 2-98% non-linear gradient |
| Flow Rate: | 0.25 ml/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS001888 |
| Analysis ID: | AN002036 |
| Instrument Name: | Agilent 6520 QTOF |
| Instrument Type: | QTOF |
| MS Type: | ESI |
| MS Comments: | Agilent MassHunter Qualitative Analysis software ultized for feature detection, Agilent Mass Profiler Professional utilized for feature filtering, and Agilent MassHunter Quantitative analysis software utilized for acquiring feature abundances. |
| Ion Mode: | POSITIVE |
| Capillary Voltage: | 4000 |
| Dry Gas Flow: | 10 l/min |
| Dry Gas Temp: | 310 |
| Fragment Voltage: | 120 |
| Nebulizer: | 45 psi |
| Octpole Voltage: | 750 V |
| Scanning Range: | 75-1700 |
| Skimmer Voltage: | 65 V |
