Return to study ST001272 main page
MB Sample ID: SA092686
Local Sample ID: | GCM_P9_2+ |
Subject ID: | SU001342 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | E18-P90 |
Weight Or Weight Range: | not measured |
Height Or Height Range: | not measured |
Gender: | Pooled |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Housing: | standard |
Animal Light Cycle: | standard 12h |
Animal Feed: | standard grain-based |
Animal Water: | standard unlimited |
Animal Inclusion Criteria: | random |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001342 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | E18-P90 |
Weight Or Weight Range: | not measured |
Height Or Height Range: | not measured |
Gender: | Pooled |
Animal Animal Supplier: | The Jackson Laboratory |
Animal Housing: | standard |
Animal Light Cycle: | standard 12h |
Animal Feed: | standard grain-based |
Animal Water: | standard unlimited |
Animal Inclusion Criteria: | random |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
GCM_P9_2+ | SA092686 | FL013269 | GCM | Fraction |
GCM_P9_2+ | SA092686 | FL013269 | P9 | Age |
Collection:
Collection ID: | CO001336 |
Collection Summary: | Growth cone from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP) were collected. These fractions were generated through established differential centrifugation techniques and lipid extraction from established protocols: 1-Estrada-Bernal, A. et al. Functional complexity of the axonal growth cone: a proteomic analysis. PloS one 7, e31858 (2012). 2-Pfenninger et al 1983 paper (Pfenninger, K. H., Ellis, L., Johnson, M. P., Friedman, L. B. & Somlo, S. Nerve growth cones isolated from fetal rat brain: subcellular fractionation and characterization. Cell 35, 573-584 (1983) |
Sample Type: | Brain |
Treatment:
Treatment ID: | TR001357 |
Treatment Summary: | No Treatment |
Sample Preparation:
Sampleprep ID: | SP001350 |
Sampleprep Summary: | We performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). |
Combined analysis:
Analysis ID | AN002112 |
---|---|
Analysis type | MS |
Chromatography type | Reversed phase |
Chromatography system | Accela 600 |
Column | Thermo Acclaim C30 (150 x 2.1mm,3m) |
MS Type | ESI |
MS instrument type | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap |
Ion Mode | UNSPECIFIED |
Units | main area |
Chromatography:
Chromatography ID: | CH001544 |
Instrument Name: | Accela 600 |
Column Name: | Thermo Acclaim C30 (150 x 2.1mm,3m) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001965 |
Analysis ID: | AN002112 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | Samples were ran in positive and negative ion mode |
Ion Mode: | UNSPECIFIED |