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MB Sample ID: SA093037
Local Sample ID: | D1_3- |
Subject ID: | SU001345 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 2 Months |
Gender: | Male and female |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001345 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6J |
Age Or Age Range: | 2 Months |
Gender: | Male and female |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
D1_3- | SA093037 | FL013316 | Sonication | Exposure |
D1_3- | SA093037 | FL013316 | Day One | Days Post Exposure |
Collection:
Collection ID: | CO001339 |
Collection Summary: | Mice were exposed to a controlled sonication pulse and optic nerve samples were collected at 1 day, 7 days, and 14 days post sonication. Lipids were then extracted, resuspended in Chloroform:Methanol (1:1) and processed through untargeted liquid chromatography Q-Exactive Orbitrap tandem mass spectrometry (LC-MS/MS). |
Sample Type: | optic nerve |
Treatment:
Treatment ID: | TR001360 |
Treatment Summary: | Mice were exposed to a 500msec sonication shock at the supraorbital rim that did not create any skull fractures nor cause death. Mice were sacrificed at 1 day, 7 days, or 14 days post exposure for lipid analysis of optic nerves. |
Sample Preparation:
Sampleprep ID: | SP001353 |
Sampleprep Summary: | As described in collection |
Combined analysis:
Analysis ID | AN002113 | AN002114 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Accela 600 | Thermo Accela 600 |
Column | Thermo Acclaim C18 (150 x 2.1mm,3m) | Thermo Acclaim C18 (150 x 2.1mm,3m) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE |
Units | Main area | Main area |
Chromatography:
Chromatography ID: | CH001547 |
Instrument Name: | Thermo Accela 600 |
Column Name: | Thermo Acclaim C18 (150 x 2.1mm,3m) |
Chromatography Type: | Reversed phase |
MS:
MS ID: | MS001968 |
Analysis ID: | AN002113 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | A Heated Electrospray Ionization Source (HESI) was operated at a spray voltage of 4.4kV, a HESI vaporization temperature of 275°C, a sheath gas pressure of 45 arbitrary units, and an auxillary gas flow of 15 arbitrary units. The ion transfer tube was kept at a temperature of 350°C. The scan range was set at 150-1500 m/z. |
Ion Mode: | POSITIVE |
MS ID: | MS001969 |
Analysis ID: | AN002114 |
Instrument Name: | Thermo Q Exactive Orbitrap |
Instrument Type: | Orbitrap |
MS Type: | ESI |
MS Comments: | A Heated Electrospray Ionization Source (HESI) was operated at a spray voltage of 4.4kV, a HESI vaporization temperature of 275°C, a sheath gas pressure of 45 arbitrary units, and an auxillary gas flow of 15 arbitrary units. The ion transfer tube was kept at a temperature of 350°C. The scan range was set at 150-1500 m/z. |
Ion Mode: | NEGATIVE |