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MB Sample ID: SA095532
Local Sample ID: | 171213_52 |
Subject ID: | SU001395 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001395 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
171213_52 | SA095532 | FL013557 | 24 h | Time |
Collection:
Collection ID: | CO001390 |
Collection Summary: | Biomass samples were withdrawn for quenching with 60% methanol. |
Sample Type: | Bacterial cells |
Treatment:
Treatment ID: | TR001410 |
Treatment Summary: | The GC/MS data were firstly converted to mzXML format and imported into R. The data were then deconvolved by using eRah package. A total number of 612 features were detected and after removing features with over 20% RSD in the QCs. |
Sample Preparation:
Sampleprep ID: | SP001403 |
Sampleprep Summary: | Metabolome were extracted from biomass snap-freezing in liquid nitrogen with 80% methanol and centrifuge at 15,000 x g -9, three times. The supernatant was dried with speed vacuum for 12 h and subsequently the samples were derivatized with methoxyamination followed by trimethylsylylation. |
Combined analysis:
Analysis ID | AN002197 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | Agilent 7200 |
Column | Agilent VF-5ms (30 m x 0.25 mm, 0.25 µm) |
MS Type | ESI |
MS instrument type | GC-TOF |
MS instrument name | Agilent 7890A |
Ion Mode | UNSPECIFIED |
Units | IU |
Chromatography:
Chromatography ID: | CH001611 |
Instrument Name: | Agilent 7200 |
Column Name: | Agilent VF-5ms (30 m x 0.25 mm, 0.25 µm) |
MS:
MS ID: | MS002043 |
Analysis ID: | AN002197 |
Instrument Name: | Agilent 7890A |
Instrument Type: | GC-TOF |
MS Type: | ESI |
MS Comments: | The mass spectrum was collected for the range of 50-550 m/z with an acquisition rate of 5 spectra/s and an acquisition time of 200 ms/spectrum |
Ion Mode: | UNSPECIFIED |