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MB Sample ID: SA098937
| Local Sample ID: | Preeclampsia_7655 |
| Subject ID: | SU001434 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001434 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| Preeclampsia_7655 | SA098937 | FL014028 | Preeclampsia | Sample_Group |
Collection:
| Collection ID: | CO001429 |
| Collection Summary: | We obtained samples from RMATRIX Hawaii Biorepository (HiBR), which obtained its own IRB approval. 44 maternal plasma samples from clinically diagnosed severe preeclampsia patients and 20 control samples (full-term deliveries without complications such as gestational diabetes) were selected. |
| Sample Type: | Blood (plasma) |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001449 |
| Treatment Summary: | We obtained samples from RMATRIX Hawaii Biorepository (HiBR), which obtained its own IRB approval. 44 maternal plasma samples from clinically diagnosed severe preeclampsia patients and 20 control samples (full-term deliveries without complications such as gestational diabetes) were selected. Plasma samples were stored at − 80 °C until the time of analysis, which was conducted by the Michigan Regional Comprehensive Metabolomics Resource Core. |
Sample Preparation:
| Sampleprep ID: | SP001442 |
| Sampleprep Summary: | Plasma samples were stored at − 80 °C until the time of analysis, which was conducted by the Michigan Regional Comprehensive Metabolomics Resource Core. Lipids were extracted using a modified BlighDyer Method. The extraction was performed using water/methanol/dichloromethane (2:2:2 v/v/v) at room temperature after the addition of internal standards. The organic layer was then collected and dried under a stream of nitrogen before being re-suspended in 100 μL of Buffer B (5%H2O:10%ACN:85%IPA containing 10 mM NH4OAc) and analyzed using a liquid chromatography tandem mass spectrometry (LC/MS/MS) lipidomics assay. The lipid extract was injected onto a 1.8 μm particle 50 × 2.1 mm internal diameter Waters Acquity HSS T3 column (Waters, Milford, MA) that was heated to 55 °C. For chromatographic elution, we used a linear gradient over a 20 min total run time. A 60% Solvent A and 40% Solvent B gradient was used for the first 10 min. Then the gradient was ramped in a linear fashion to 100% Solvent B which was maintained for 7 min. Thereafter, the system was switched back to 60% Solvent B and 40% Solvent A for 3 min. The flow rate used for these experiments was 0.4 mL/min and the injection volume was 5 μL. The column was equilibrated for 3 min before the next injection and run at a flow rate of 0.400uL/min for a total run time of 20 min. Data were acquired in positive and negative modes using data-dependent MS/MS with dynamic mass exclusion. Pooled human plasma samples and pooled experimental samples (prepared by combining small aliquots of each experimental sample) were used to control for the quality of sample preparation and analysis. A randomization scheme was used to distribute pooled samples within the set and a mixture of pure authentic standards was used to monitor instrument performance on a regular basis. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | Described in summary |
Combined analysis:
| Analysis ID | AN002264 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity H-Class |
| Column | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | Ion trap |
| MS instrument name | Waters Micromass QTOF Premier |
| Ion Mode | UNSPECIFIED |
| Units | normalized |
Chromatography:
| Chromatography ID: | CH001663 |
| Instrument Name: | Waters Acquity H-Class |
| Column Name: | Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) |
| Chromatography Type: | HILIC |
MS:
| MS ID: | MS002108 |
| Analysis ID: | AN002264 |
| Instrument Name: | Waters Micromass QTOF Premier |
| Instrument Type: | Ion trap |
| MS Type: | ESI |
| MS Comments: | Lipids were identified using the LIPIDBLAST computer-generated tandem MS library. This database contains 212,516 spectra covering 119,200 compounds representing 26 lipid classes, including phospholipids, glycerolipids, bacterial lipoglycan, and plant glycolipids. Quantification of lipids was completed using ABSCIEX MultiQuant software. Compounds with a relative standard deviation greater than 30% in the pooled samples were excluded. |
| Ion Mode: | UNSPECIFIED |
