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MB Sample ID: SA099572

Local Sample ID:KAS5321.fullMS.neg
Subject ID:SU001444
Subject Type:Fungi
Subject Species:Alternaria sp.
Taxonomy ID:1715220

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Subject:

Subject ID:SU001444
Subject Type:Fungi
Subject Species:Alternaria sp.
Taxonomy ID:1715220

Factors:

Local Sample IDMB Sample IDFactor Level IDLevel ValueFactor Name
KAS5321.fullMS.negSA099572FL0140761Group
KAS5321.fullMS.negSA099572FL014076Lycopersicon esculentumHost Plant

Collection:

Collection ID:CO001439
Collection Summary:The majority of fungal isolates were obtained through the Canadian Collection of Fungal Cultures (CCFC) at the Agriculture and Agri-Food Canada in Ottawa, Ontario Canada. Strains from tomato in London, Ontario were isolated by putting tomato stems and fruit in moist chambers, and aseptically transferring Alternaria hypahe and spores after genus level verification under a dissecting microscope. Nova Scotia and Vineland grape and blueberry material were collected similarly.
Sample Type:Plant
Collection Location:London, Ontario; Ottawa, Ontario; Mt. Thom, Nova Scotia; Vineland, Ontario; P.E.I.; Quebec; Dunmore, Alberta; Edmonton, Alberta; Killam, Alberta; Beechy, Saskatchewan; Corrine, Saskatchewan;
Storage Conditions:-20℃

Treatment:

Treatment ID:TR001459
Treatment Summary:All fungal strains were grown as three point inoculations on potato dextrose agar (PDA) in the dark at 25°C for seven days, or until colonies reached approximately 4 cm in diameter.

Sample Preparation:

Sampleprep ID:SP001452
Sampleprep Summary:Cultures were extracted by removing six agar plugs from both the centre and edges of the three point inoculations using a 6 mm cork borer. Agar plugs were extracted with ethyl acetate containing 1% formic acid. Extracts were dried under nitrogen, and reconstituted with acetonitrile before filtration by 0.45µm PTFE syringe filters.

Combined analysis:

Analysis ID AN002286 AN002287
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity
Column Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm) Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Integrated peak area Integrated peak area

Chromatography:

Chromatography ID:CH001680
Chromatography Summary:LC-MS conditions for negative and positive ionization modes
Instrument Name:Agilent 1290 Infinity
Column Name:Agilent ZORBAX RRHD Eclipse Plus C18 (50 x 2.1 mm,1.8 µm)
Column Temperature:35
Flow Gradient:0%B held for 0.5 minutes, increased to 100%B over 3.5 minutes. Hold B at 100% for 1.5 minutes, and then return to 0%B over 0.5 minutes
Flow Rate:0.3 mL/min
Sample Injection:2 µL
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Capillary Voltage:(-) 3.7 kV; (+) 3.9 kV
Chromatography Type:Reversed phase

MS:

MS ID:MS002130
Analysis ID:AN002286
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Data were acquired in full MS-SIM mode at 140,000 resolution over a m/z range of 100-1200
Ion Mode:POSITIVE
Capillary Temperature:400°C
Capillary Voltage:3.9 kV
Automatic Gain Control:1e6
  
MS ID:MS002131
Analysis ID:AN002287
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Data were acquired in full MS-SIM mode at 140,000 resolution over a m/z range of 100-1200
Ion Mode:NEGATIVE
Capillary Temperature:400°C
Capillary Voltage:3.7 kV
Automatic Gain Control:1e6
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