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MB Sample ID: SA100494
Local Sample ID: | WT_7 |
Subject ID: | SU001449 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | HepG2 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001449 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Genotype Strain: | HepG2 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
WT_7 | SA100494 | FL014158 | WT | Genotype |
Collection:
Collection ID: | CO001444 |
Collection Summary: | Cell cultures for both cell lines were grown simultaneously with identical media components. Upon achieving ~80% confluence in 10cm culture dish, culture media was removed and cell monolayer was washed with cold phosphate buffered saline. Cells were then quenched by addition ice-cold 80% methanol extraction solvent, scraped from the culture dish, and flash-frozen in liquid nitrogen. |
Sample Type: | HepG2 cells |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001464 |
Treatment Summary: | None |
Sample Preparation:
Sampleprep ID: | SP001457 |
Sampleprep Summary: | Aqueous metabolites were extracted by vortexing cell pellets in the extraction solvent, pelleting cell debris and collecting the supernatant. Approximately 10% of the supernatant volume was taken from each sample to form an internal pooled sample. The solvent was then evaporated to produce dried extracts. |
Sampleprep Protocol Filename: | cell_protocol.pdf |
Processing Storage Conditions: | On ice |
Extraction Method: | Vortex |
Extract Enrichment: | Polar |
Extract Storage: | -80℃ |
Sample Resuspension: | Deuterium oxide phosphate buffer |
Analysis:
MB Sample ID: | SA100494 |
Analysis ID: | AN002295 |
Laboratory Name: | Edison |
Analysis Type: | NMR |
Acquisition Date: | 22-Feb-18 |
Software Version: | Topspin 4.0.1 |
Operator Name: | Maxwell Colonna |
Data Format: | Bruker |
Num Factors: | 2 |
Num Metabolites: | 24 |
Units: | AU |
NMR:
NMR ID: | NM000164 |
Analysis ID: | AN002295 |
Instrument Name: | Bruker Avance |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | Deuterium |
Spectrometer Frequency: | 800 MHz |
NMR Probe: | 3mm Cryoprobe |
NMR Solvent: | D2O Phosphate buffer |
NMR Tube Size: | 3mm SampleJet |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | Presaturation |
Pulse Width: | 8.22 us |
Power Level: | 11.117 W |
Offset Frequency: | 3762.64 |
Presaturation Power Level: | 3.00E-05 |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 27 |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 1.24 s |
Relaxation Delay: | 2 s |
Spectral Width: | 16.4533 ppm |
Num Data Points Acquired: | 32768 |
Line Broadening: | 0.3 Hz |
Baseline Correction Method: | Polynomial order 5 |