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MB Sample ID: SA100499
| Local Sample ID: | WT_5 |
| Subject ID: | SU001449 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | HepG2 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001449 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Genotype Strain: | HepG2 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| WT_5 | SA100499 | FL014158 | WT | Genotype |
Collection:
| Collection ID: | CO001444 |
| Collection Summary: | Cell cultures for both cell lines were grown simultaneously with identical media components. Upon achieving ~80% confluence in 10cm culture dish, culture media was removed and cell monolayer was washed with cold phosphate buffered saline. Cells were then quenched by addition ice-cold 80% methanol extraction solvent, scraped from the culture dish, and flash-frozen in liquid nitrogen. |
| Sample Type: | HepG2 cells |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001464 |
| Treatment Summary: | None |
Sample Preparation:
| Sampleprep ID: | SP001457 |
| Sampleprep Summary: | Aqueous metabolites were extracted by vortexing cell pellets in the extraction solvent, pelleting cell debris and collecting the supernatant. Approximately 10% of the supernatant volume was taken from each sample to form an internal pooled sample. The solvent was then evaporated to produce dried extracts. |
| Sampleprep Protocol Filename: | cell_protocol.pdf |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Vortex |
| Extract Enrichment: | Polar |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Deuterium oxide phosphate buffer |
Analysis:
| MB Sample ID: | SA100499 |
| Analysis ID: | AN002295 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Acquisition Date: | 22-Feb-18 |
| Software Version: | Topspin 4.0.1 |
| Operator Name: | Maxwell Colonna |
| Data Format: | Bruker |
| Num Factors: | 2 |
| Num Metabolites: | 24 |
| Units: | AU |
NMR:
| NMR ID: | NM000164 |
| Analysis ID: | AN002295 |
| Instrument Name: | Bruker Avance |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Spectrometer Frequency: | 800 MHz |
| NMR Probe: | 3mm Cryoprobe |
| NMR Solvent: | D2O Phosphate buffer |
| NMR Tube Size: | 3mm SampleJet |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | Presaturation |
| Pulse Width: | 8.22 us |
| Power Level: | 11.117 W |
| Offset Frequency: | 3762.64 |
| Presaturation Power Level: | 3.00E-05 |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 27 |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 1.24 s |
| Relaxation Delay: | 2 s |
| Spectral Width: | 16.4533 ppm |
| Num Data Points Acquired: | 32768 |
| Line Broadening: | 0.3 Hz |
| Baseline Correction Method: | Polynomial order 5 |
