Return to study ST001397 main page
MB Sample ID: SA113563
| Local Sample ID: | L_K9D14_neg |
| Subject ID: | SU001471 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001471 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| L_K9D14_neg | SA113563 | FL014330 | 9 | Litter |
| L_K9D14_neg | SA113563 | FL014330 | 14 | Day after birth |
Collection:
| Collection ID: | CO001466 |
| Collection Summary: | Animals were sacrificed and the liver tissue was removed, snap frozen in liquid nitrogen and stored at -80°C until further analysis |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001486 |
| Treatment Summary: | All C57BL6J wildtype mice were bred locally and held under specific pathogen-free conditions at the Institute of Laboratory Animal Science at RWTH Aachen University Hospital. The day of birth was considered day 0, i.e. animals screened at day 1 were approximately 24 h old and verified to have ingested breast milk (abdominal milk spot). Mice were weaned at PND21. |
Sample Preparation:
| Sampleprep ID: | SP001479 |
| Sampleprep Summary: | Sample preparation for MetIDQ p180 Kit measurement Solvents: Acetonitril (Merck KGaA, Darmstadt, Germany hypergrade for LC-MS) Water MiliQ, Extracting agent - ACN / H2O (1:1) Equipment 4 steel balls size M Eppendorf Tubes 2mL Tissue slicer (Rettberg, Germany) Centrifuge (Sigma) Work steps Approximately 100mg of sample and 4 steel balls of size M into each tube. Per mg of sample 5µL of extracting agent was added. Shake the samples for 10 minutes at 30 Hz in the tissue slicer and centrifuge for 2 minutes at 14000 rpm. The supernatant was used for the targeted analytics. Kit reparation The analysis was performed using the validated MetIDQ p180 Kit and described in Siskos et al. [1]. Data processing was carried out with the provided quantitation method Kit (Biocrates Life Sciences AG, Innsbruck, Austria). 1 Siskos AP, Jain P, Römisch-Margl W, Bennett M, Achaintre D, Asad Y, et al. Interlaboratory Reproducibility of a Targeted Metabolomics Platform for Analysis of Human Serum and Plasma. Anal Chem 2017;89:656-65. |
Combined analysis:
| Analysis ID | AN002336 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | µM |
Chromatography:
| Chromatography ID: | CH001712 |
| Instrument Name: | UPLC (Waters Acquity, Waters Corporation, Milford, USA) |
| Column Name: | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002178 |
| Analysis ID: | AN002336 |
| Instrument Name: | ABI Sciex 5500 QTrap |
| Instrument Type: | Triple quadrupole |
| MS Type: | ESI |
| MS Comments: | Analyst version 1.6 Software from SCIEX. For Validation METIDQ Software version Boron from Biocrates |
| Ion Mode: | NEGATIVE |
