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MB Sample ID: SA121887
| Local Sample ID: | B18_04 |
| Subject ID: | SU001508 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
| Subject ID: | SU001508 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| B18_04 | SA121887 | FL014906 | B1.8 | Antibody Group |
Collection:
| Collection ID: | CO001503 |
| Collection Summary: | Liver were collected after mice sacrifice, frozen in liquid N2 and store at -80°C until the day of analysis. |
| Sample Type: | Liver |
Treatment:
| Treatment ID: | TR001523 |
| Treatment Summary: | LDLR-/-mice under high fat diet were injected with mA12-IgG2b and PBS or a B1-8 IgG2b isotype as control antibody |
Sample Preparation:
| Sampleprep ID: | SP001516 |
| Sampleprep Summary: | Frozen liver tissues were lysed in 0.5X PBS (sample:solvent 1:10 ratio) containing 1 mg/mL butylhydroxytoluene (BHT) solution in methanol (proportion 200 µL per g of tissue), with FastPrep-24 5G instrument (MP Biomedicals, USA). 25 µL aliquots of homogenate were collected and stored at -80°C until lipidomic extraction. Tissue homogenates were thawed and extracted with methyl-tert-butylether (MTBE) as described in [Matyash, V., et al., Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of lipid research, 2008. 49(5): p. 1137-46]. 900 µL of organic phase were dried-out in speedvac and resuspended in 50 µL of ACN:H2O (20:80, v:v) just before injection. |
Combined analysis:
| Analysis ID | AN002396 | AN002397 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 | Thermo Dionex Ultimate 3000 |
| Column | Agilent mRP-Recovery C18 column (100 x 0.5mm,5um) | Agilent mRP-Recovery C18 column (100 x 0.5mm,5um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH001761 |
| Instrument Name: | Thermo Dionex Ultimate 3000 |
| Column Name: | Agilent mRP-Recovery C18 column (100 x 0.5mm,5um) |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS002237 |
| Analysis ID: | AN002396 |
| Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS was operating in full scan mode from 70 to 1700 m/z at 60000 resolution. Data processing was carried-out using Compound Discoverer (ThermoFisher; USA) with the Metaboprofiler node. |
| Ion Mode: | POSITIVE |
| MS ID: | MS002238 |
| Analysis ID: | AN002397 |
| Instrument Name: | Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap |
| Instrument Type: | Orbitrap |
| MS Type: | ESI |
| MS Comments: | MS was operating in full scan mode from 70 to 1700 m/z at 60000 resolution. Data processing was carried-out using Compound Discoverer (ThermoFisher; USA) with the Metaboprofiler node. |
| Ion Mode: | NEGATIVE |
