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MB Sample ID: SA123775
Local Sample ID: | 594 |
Subject ID: | SU001522 |
Subject Type: | Mammal |
Subject Species: | Ovis aries |
Taxonomy ID: | 9940 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001522 |
Subject Type: | Mammal |
Subject Species: | Ovis aries |
Taxonomy ID: | 9940 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
594 | SA123775 | FL014990 | Control | Treatment |
594 | SA123775 | FL014990 | Male | Sex |
Collection:
Collection ID: | CO001517 |
Collection Summary: | Placentomes were collected at necropsy from control ewes and ewes treated with cortisol (CORT: 1mg/kg/d from day 115 of pregnancy) at days 141-144 of gestation. CORT ewes received an intravenous infusion of cortisol (Solu-Cortef, hydrocortisone sodium succinate in sodium phosphate; Pfizer, New York, NY); control animals did not receive any treatment. Animals were sacrificed for collection of tissues at the first signs of labor, as evidenced by changes in uterine blood flow; placentomes were collected from sheep in early stages of labor at between 141-144 days and 142-144 days of gestation in the cortisol-treated and control groups, respectively. In this cohort of ewes, there were 7 control ewes, of which data were collected from placentomes of 7 live fetuses (including one set of twins); there was one lamb born, and that placenta was not collected. In this cohort there were 11 cortisol- treated ewes, from which we collected placentomes from 5 live fetuses. There was one live lamb born, and 5 stillborn lambs, from which placentas were not collected. Placentomes were classified as A-D placentomes, snap frozen in liquid nitrogen, and stored in -80C until metabolomic analysis. |
Sample Type: | Placenta |
Treatment:
Treatment ID: | TR001537 |
Treatment Summary: | Placentomes were collected at necropsy from control ewes and ewes treated with cortisol (CORT: 1mg/kg/d from day 115 of pregnancy) at days 141-144 of gestation. CORT ewes received an intravenous infusion of cortisol (Solu-Cortef, hydrocortisone sodium succinate in sodium phosphate; Pfizer, New York, NY); control animals did not receive any treatment. |
Sample Preparation:
Sampleprep ID: | SP001530 |
Sampleprep Summary: | High-resolution magic angle spinning (HR-MAS) proton nuclear magnetic resonance (1H-NMR) was used to determine metabolites present in placental tissue. Briefly, 30 μL of deuterium oxide (D2O) with 3.3 mM 3-(Trimethylsilyl)-1-propanesulfonic acid (DSS) as a reference standard was added to 27.7-52.1 mg (mean 35.3 mg) of placental tissue before being placed into a 4 mm HR-MAS rotor (Bruker Biospin) and spun at 6 kHz with a spectral width of 10 ppm. Data were acquired on an Avance III 600 MHz Bruker NMR spectrometer equipped with a 4 mm HR-MAS probe at the University of Georgia Complex Carbohydrate Research Center. Data were acquired using a one-dimensional experiment with T2 filter using Carr-Purcell-Meiboom-Gill (CPMG) pulse sequence with water presaturation (CPMGPR1D). |
Analysis:
MB Sample ID: | SA123775 |
Analysis ID: | AN002419 |
Num Factors: | 3 |
Num Metabolites: | 38 |
Units: | Area Under Curve |
NMR:
NMR ID: | NM000169 |
Analysis ID: | AN002419 |
Instrument Name: | Bruker Avance III HD |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Spectrometer Frequency: | 600 MHz |