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MB Sample ID: SA124132
Local Sample ID: | 06_B2_Liver_syngenic_42days_170427_UKy_GCH_rep3-polar-ICMS_A |
Subject ID: | SU001527 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001527 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
06_B2_Liver_syngenic_42days_170427_UKy_GCH_rep3-polar-ICMS_A | SA124132 | FL015007 | syngenic | Treatment Protocol |
Collection:
Collection ID: | CO001522 |
Collection Summary: | Mouse is sacrificed and tissues are harvested. |
Collection Protocol ID: | mouse_tissue_collection |
Sample Type: | Multiple tissues |
Treatment:
Treatment ID: | TR001542 |
Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
Sampleprep ID: | SP001535 |
Sampleprep Summary: | Tissue is frozen in liquid nitrogen to stop metabolic processes. Polar extraction from homogenate, lypholized, and frozen. Protein extraction and quantification. Before going into the IC-FTMS the frozen sample is reconstituted in water. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. |
Sampleprep Protocol ID: | frozen_tissue_grind; IC-FTMS_preparation; protein_extraction; polar_extraction; tissue_quench |
Sampleprep Protocol Filename: | 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
Combined analysis:
Analysis ID |
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Analysis type |
Chromatography type |
Chromatography system |
Column |
MS Type |
MS instrument type |
MS instrument name |
Ion Mode |
Units |
Chromatography:
Chromatography ID: | CH001785 |
Chromatography Summary: | Targeted IC |
Instrument Name: | Thermo Dionex ICS-5000+ |
Column Name: | Dionex IonPac AS11-HC (250 x 2mm,4um) |
Chromatography Type: | Ion exchange |
MS: