Return to study ST001469 main page
MB Sample ID: SA124494
Local Sample ID: | 23_C1-2_Lung_allogenic_42days_170427_UKy_GCH_rep2-lipid |
Subject ID: | SU001543 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Select appropriate tab below to view additional metadata details:
Subject:
Subject ID: | SU001543 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
23_C1-2_Lung_allogenic_42days_170427_UKy_GCH_rep2-lipid | SA124494 | FL015062 | allogenic | Treatment Protocol |
Collection:
Collection ID: | CO001538 |
Collection Summary: | Mouse is sacrificed and tissues are harvested. |
Collection Protocol ID: | mouse_tissue_collection |
Sample Type: | Multiple tissues |
Treatment:
Treatment ID: | TR001558 |
Treatment Summary: | Mouse with allogenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with no treatment. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. Mouse with syngenic bone marrow transplant. Fed with semi-liquid diet supplemented with fully labeled glucose for 24 hours before harvest. |
Treatment Protocol ID: | allogenic naive syngenic |
Sample Preparation:
Sampleprep ID: | SP001551 |
Sampleprep Summary: | Tissue is frozen in liquid nitrogen to stop metabolic processes. Lipid extraction from homogenate. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. Protein extraction and quantification. |
Sampleprep Protocol ID: | frozen_tissue_grind; lipid_extraction; protein_extraction; tissue_quench |
Sampleprep Protocol Filename: | 4D_17Jun4_Fan_Prot_Quant.pdf 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf |
Combined analysis:
Analysis ID | AN002445 |
---|---|
Analysis type | MS |
Chromatography type | None (Direct infusion) |
Chromatography system | None |
Column | None |
MS Type | ESI |
MS instrument type | FTMS |
MS instrument name | Orbitrap Fusion |
Ion Mode | POSITIVE |
Units | peak_height |
Chromatography:
Chromatography ID: | CH001790 |
Chromatography Summary: | None |
Instrument Name: | None |
Column Name: | None |
Chromatography Type: | None (Direct infusion) |
MS:
MS ID: | MS002268 |
Analysis ID: | AN002445 |
Instrument Name: | Orbitrap Fusion |
Instrument Type: | FTMS |
MS Type: | ESI |
MS Comments: | Measurements made using direct infusion (nano-electrospray) FTMS. |
Ion Mode: | POSITIVE |