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MB Sample ID: SA124712
Local Sample ID: | batch1_121 |
Subject ID: | SU001550 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | Astarte Bio |
Cell Strain Details: | Cat 1017-3975JY18 |
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Subject:
Subject ID: | SU001550 |
Subject Type: | Cultured cells |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | Astarte Bio |
Cell Strain Details: | Cat 1017-3975JY18 |
Factors:
Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
---|---|---|---|---|
batch1_121 | SA124712 | FL015161 | DMS | method |
batch1_121 | SA124712 | FL015161 | 4 | timepoint |
batch1_121 | SA124712 | FL015161 | 10 | il2_conc |
batch1_121 | SA124712 | FL015161 | 2500 | carrier_conc |
batch1_121 | SA124712 | FL015161 | 100 | abs_surface_density |
Collection:
Collection ID: | CO001545 |
Collection Summary: | Conditioned culture media (Miltenyi Texmacs media). Was collected via pipetting as described in sample prep protocol document. |
Sample Type: | T-cells |
Collection Method: | Pipette |
Volumeoramount Collected: | 60 uL |
Storage Conditions: | -80℃ |
Treatment:
Treatment ID: | TR001565 |
Treatment Summary: | Cultures were prepared with varied IL2 concentration, DMS concentration, and DMS surface densities as described in study design. Culture media was replenished on days 4, 6, 8, 11 after sampling. |
Cell Media: | Miltenyi Texmacs (Cat. 170-076-307) |
Sample Preparation:
Sampleprep ID: | SP001558 |
Sampleprep Summary: | Media samples were thawed at 4°C, then centrifuged at maximum speed for 15 minutes. 45 uL were transferred directly to NMR tube containing 5 uL of D2O with 100 uM DSS and mixed. Remaining supernatant from each sample combined for internal controls. |
Sampleprep Protocol Filename: | mbcolonna_20200812_100701_PR_SP_protocol.pdf |
Processing Storage Conditions: | 4℃ |
Extraction Method: | None |
Analysis:
MB Sample ID: | SA124712 |
Analysis ID: | AN002452 |
Laboratory Name: | Edison |
Analysis Type: | NMR |
Operator Name: | Maxwell Colonna |
Data Format: | Bruker |
Num Factors: | 183 |
Rt Units: | No RT Data |
Results File: | ST001476_AN002452_Results.txt |
Units: | UNITS:AU |
NMR:
NMR ID: | NM000183 |
Analysis ID: | AN002452 |
Instrument Name: | Bruker Avance III HD |
Instrument Type: | FT-NMR |
NMR Experiment Type: | 1D-1H |
Field Frequency Lock: | Deuterium |
Spectrometer Frequency: | 600 MHz |
NMR Probe: | 5mm TCI |
NMR Solvent: | 90% H2O, 10% D2O |
NMR Tube Size: | 1.7mm |
Shimming Method: | Topshim |
Pulse Sequence: | noesypr1d |
Water Suppression: | Presaturation |
Pulse Width: | 7.45 us |
Power Level: | 5.5584 W |
Receiver Gain: | 203 |
Offset Frequency: | 2819.9 Hz |
Presaturation Power Level: | 1.2333e-05 W |
Chemical Shift Ref Cpd: | DSS |
Temperature: | 25 |
Number Of Scans: | 128 |
Dummy Scans: | 4 |
Acquisition Time: | 2.72 s |
Relaxation Delay: | 4 s |
Spectral Width: | 20 ppm |
Num Data Points Acquired: | 65382 |
Line Broadening: | 0.3 Hz |
Baseline Correction Method: | Polynomial order 5 |