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MB Sample ID: SA124764
| Local Sample ID: | batch1_44 |
| Subject ID: | SU001550 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | Astarte Bio |
| Cell Strain Details: | Cat 1017-3975JY18 |
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Subject:
| Subject ID: | SU001550 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | Astarte Bio |
| Cell Strain Details: | Cat 1017-3975JY18 |
Factors:
| Local Sample ID | MB Sample ID | Factor Level ID | Level Value | Factor Name |
|---|---|---|---|---|
| batch1_44 | SA124764 | FL015207 | DMS | method |
| batch1_44 | SA124764 | FL015207 | 6 | timepoint |
| batch1_44 | SA124764 | FL015207 | 30 | il2_conc |
| batch1_44 | SA124764 | FL015207 | 1500 | carrier_conc |
| batch1_44 | SA124764 | FL015207 | 80 | abs_surface_density |
Collection:
| Collection ID: | CO001545 |
| Collection Summary: | Conditioned culture media (Miltenyi Texmacs media). Was collected via pipetting as described in sample prep protocol document. |
| Sample Type: | T-cells |
| Collection Method: | Pipette |
| Volumeoramount Collected: | 60 uL |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR001565 |
| Treatment Summary: | Cultures were prepared with varied IL2 concentration, DMS concentration, and DMS surface densities as described in study design. Culture media was replenished on days 4, 6, 8, 11 after sampling. |
| Cell Media: | Miltenyi Texmacs (Cat. 170-076-307) |
Sample Preparation:
| Sampleprep ID: | SP001558 |
| Sampleprep Summary: | Media samples were thawed at 4°C, then centrifuged at maximum speed for 15 minutes. 45 uL were transferred directly to NMR tube containing 5 uL of D2O with 100 uM DSS and mixed. Remaining supernatant from each sample combined for internal controls. |
| Sampleprep Protocol Filename: | mbcolonna_20200812_100701_PR_SP_protocol.pdf |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | None |
Analysis:
| MB Sample ID: | SA124764 |
| Analysis ID: | AN002452 |
| Laboratory Name: | Edison |
| Analysis Type: | NMR |
| Operator Name: | Maxwell Colonna |
| Data Format: | Bruker |
| Num Factors: | 183 |
| Rt Units: | No RT Data |
| Results File: | ST001476_AN002452_Results.txt |
| Units: | UNITS:AU |
NMR:
| NMR ID: | NM000183 |
| Analysis ID: | AN002452 |
| Instrument Name: | Bruker Avance III HD |
| Instrument Type: | FT-NMR |
| NMR Experiment Type: | 1D-1H |
| Field Frequency Lock: | Deuterium |
| Spectrometer Frequency: | 600 MHz |
| NMR Probe: | 5mm TCI |
| NMR Solvent: | 90% H2O, 10% D2O |
| NMR Tube Size: | 1.7mm |
| Shimming Method: | Topshim |
| Pulse Sequence: | noesypr1d |
| Water Suppression: | Presaturation |
| Pulse Width: | 7.45 us |
| Power Level: | 5.5584 W |
| Receiver Gain: | 203 |
| Offset Frequency: | 2819.9 Hz |
| Presaturation Power Level: | 1.2333e-05 W |
| Chemical Shift Ref Cpd: | DSS |
| Temperature: | 25 |
| Number Of Scans: | 128 |
| Dummy Scans: | 4 |
| Acquisition Time: | 2.72 s |
| Relaxation Delay: | 4 s |
| Spectral Width: | 20 ppm |
| Num Data Points Acquired: | 65382 |
| Line Broadening: | 0.3 Hz |
| Baseline Correction Method: | Polynomial order 5 |
